Anti-nutritional factors in cowpea cultivars and their effects on susceptibility to Callosobruchus maculatus (Fab.) [Coleoptera: Bruchidae] infestation
Kayode David Ileke
Department of Environmental Biology and Fisheries, Faculty of Science, Adekunle Ajasin University, PMB 001, Akungba Akoko, Ondo State, Nigeria
Bioscience Methods, 2014, Vol. 5, No. 2 doi: 10.5376/bm.2014.05.0002
Received: 30 Mar., 2014 Accepted: 08 Sep., 2014 Published: 20 Nov., 2014
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Preferred citation for this article:
Ileke, 2014, Anti-nutritional factors in cowpea cultivars and their effects on susceptibility to Callosobruchus maculatus (Fab.) [Coleoptera: Bruchidae] infestation, Bioscience Methods, Vol.5, No.2 1-8 (doi: 10.5376/bm.2014.05.0002)
Thirty-one cowpea cultivars from IITA in Nigeria were investigated for their antinutritional factors to determine their susceptibility to cowpea bruchid, Callosobruchus maculatus (Fab.) [Coleoptera: Chrysomelidae] infestation. Percentage seed damage and pest tolerance, Phytate, tannins and oxalate contents were determined using standard methods. The result showed significant variations in both % seed damage and pest tolerance among the cowpea cultivars tested. Cultivars MIT04K-399-1, MIT07K-299-92, IT96-610, MIT06K-281-1, MIT07K-187-24, MIT06K-121, MIT07K-304-9 and EIT07K-291-69 were the least susceptible to C. maculatus with 100% pest tolerance. The result of anti-nutritional factors clearly showed that cowpea seeds contain more phytate than oxalate and tannin. The mean phytate content in seeds was (3.10 mg/g) which was about three times higher than that of oxalate (0.78 mg/g) and five times higher than tannins (0.31 mg/g). Cultivar MIT04K-339-1 recorded the highest amount of phytate while cultivar MIT03K-337-6 recorded the lowest. The amount of oxalate ranges from 0.42 mg/g to 0.92 mg/g. Cultivar IT96-610 recorded the highest amount of tannin while cultivar MIT03K-337-6 recorded the lowest amount of tannin. This study revealed variations in the anti-nutrients composition among the cowpea cultivars. These variations contribute to the susceptibility of cowpea to C. maculatus infestation.
Callosobruchus maculatus; Cowpea seeds; Pest tolerance; Phytate; tannins; Oxalate; Susceptibility indices