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Studies on Construction of Regeneration System and Genetic Transformation of Puccinellia chinampoensis | Wang 1 | Bioscience Methods

Studies on Construction of Regeneration System and Genetic Transformation of Puccinellia chinampoensis  

Tao Wang1 , Xue Han1 , Mengqing Zhao1 , Takano Tetsuo2 , Shenkui Liu1,2
1. Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field (SAVER), Ministry of Education, Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, Harbin, 150040, P.R. China;
2. Asian Natural Environmental Science Center(ANESC), University of Tokyo, 1-1-1 Yayoi, Bunkyo-ku, Tokyo, 188-0002, Japan
Author    Correspondence author
Bioscience Methods, 2011, Vol. 2, No. 5   doi: 10.5376/bm.2011.02.0005
Received: 02 Jun., 2011    Accepted: 20 Jun., 2011    Published: 23 Jun., 2011
© 2011 BioPublisher Publishing Platform
This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Wang et al., 2011, Studies on Construction of Regeneration System and Genetic Transformation of Puccinellia chinampoensis, Bioscience Methods, doi:10.5376/bm.2011.02.0005

Abstract

The regeneration system was established and the genetic transformation was processed, using the mature seeds of Puccinellia chinampoensis as explants. The results showed that the callus induction rate could reach to 39.75% after 19days of induction with the altered mediumâ…  (adding 4 mg/L 2,4-D, 500 mg/L proline and 500 mg/L glutamine) (pH 5.8~6.0). When using the modified medium S (adding 2 mg/L 2,4-D, 1 mg/L ABA, 500 mg/L proline and 600 mg/L caseinhydrolysate) (pH 5.8~6.0) as the subculture medium, we can get much more embryogenic callus. The differentiation rate could reach to 53.73% using the differentiation medium (MS as the basic medium, adding 0.4 mg/L ABA, 0.04 mg/L IAA, 500 mg/L proline and 600 mg/L caseinhydrolysate) (pH 5.8~6.0). On the genetic transformation, the transformation efficiency was best when the infect time lasted 30 min, making using of the Agrobacterium tumefaciens mediated pBI121-GUS transformation when OD600 reached to 0.8~1.0.

 

Keywords
Puccinellia chinampoensis; Regeneration system; Genetic transformation
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