Callus Induction and Plant Regeneration from Rosemary Leaves
1. College of Agronomy and Biotechnology, Yunnan Agricultural University, Kunming, 650201, P.R. China;
2. Hubei Key Laboratory of Economic Forest Germplasm Improvement and Resource Comprehensive Utilization, Huanggang Normal University, Huanggang, 438000, P.R. China
3. Science and technology training center of Kunming, Kunming, 650021, P.R. China
* These authors contributed equally to this work
Bioscience Methods, 2012, Vol. 3, No. 3 doi: 10.5376/bm.2012.03.0003
Received: 05 Mar., 2012 Accepted: 03 May, 2012 Published: 08 May, 2012
© 2012 BioPublisher Publishing Platform
This article was first published in Molecular Plant Breeding (Vol.10, No.2, 189-194) in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:
Dong et al., 2012, Callus Induction and Plant Regeneration from Rosemary Leaves, Bioscience Methods, Vol.3, No.3 21-26 (doi: 10.5376/bm.2012.03.0003)
Rosmarinus officinalis L. leaves were used as explants in the study to investigate factors affecting callus induction and plant regeneration. It was found that the culture medium with higher concentration of sucrose promoted callus induction. MS medium supplemented with 6-BA 0.5 mg/L, NAA 0.5 mg/L, and sucrose 50 g/L showed the best result with an induction rate of 88.8%. When regenerated callus develops shoots, it was found that MS medium containing 6-BA 1.5 mg/L, KT 0.5 mg/L and NAA 0.5 mg/L showed relative good result with 50% regeneration rate. When grown in 6-BA 0.8 mg/L and NAA 0.5 mg/L, the amount of growth was increased by more than 300%. MS medium containing NAA 0.1 mg/L showed better result in inducing roots with rooting rate reaching 65%. Additionally, the study also showed that even though the explants were sterile, solution containing 75% ethanol with other sterilizing reagents would result in mass explant deaths.
Rosmarinus officinalis L.; Leaf explant; Callus; Differentiation; Regeneration