Protocol for the fast isolation and identification of insecticidal Bacillus thuringiensis strains from soil  

Palma L.1,2
1.Facultad de Ciencias Agrarias, Universidad Nacional del Litoral, Esperanza, Santa Fe, Argentina
2.Consejo Nacional de Investigaciones Científicas y Técnicas, Argentina
Author    Correspondence author
Bt Research, 2015, Vol. 6, No. 7   doi: 10.5376/bt.2015.06.0007
Received: 19 Aug., 2015    Accepted: 20 Sep., 2014    Published: 08 Nov., 2015
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This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Palma L 2015, Protocol for the fast isolation and identification of insecticidal Bacillus thuringiensis strains from soil, Bt Research, Vol.6, No.7, 1-3 (doi: 10.5376/bt.2015.06.0007)

Abstract

Bacillus thuringiensis (Bt) is an ubiquitous, Gram-positive and sporulating bacterium that synthesizes insecticidal proteins with specificity against a wide range of insects during sporulation (Cry and Cyt) and vegetative growth (Vip and Sip). These proteins have portrayed Bt as an environment-friendly alternative to chemical insecticides. However, the intensive use of this resource has promoted the evolution of insect resistance to some of the most used Bt strains to date. Continuous efforts are needed to obtain novel strains exhibiting new specificities in order to overcome insect resistance and maintaining the insecticidal potential of this bacterium. In this work, an experimental procedure for the fast isolation and identification of insecticidal Bt strains is described.

Keywords
Bacillus thuringiensis (Bt); Bt toxins; biological control
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