Draft Genome Sequence of Bacillus thuringiensis Strain S2685-1, a Novel Strain with High Larvacidal Toxicity against Helicoverpa zea
1 Hainan Institute of Tropical Agricultural Resources (HITAR), Sanya, China
2 College of Life and Technology Science, Guangxi University, Nanning, Guangxi, China
3 Alkali Soil Natural Environmental Science Center(ASNESC), Northeast Forestry University, Harbin, 150040, China
4 College of Bioinformatics Science and Technology, Harbin Medical University, Harbin, China
5 College of Life Sciences, Hainan Normal University, Haikou, China
6 The HITAR Institute Canada Inc. British Columbia, Canada
* These authors contributed equally.
Bt Research, 2015, Vol. 6, No. 9 doi: 10.5376/bt.2015.06.0009
Received: 30 Oct., 2015 Accepted: 20 Nov., 2015 Published: 16 Dec., 2015
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Preferred citation for this article:
Zhou Y., Wu Z.Q., Liu P.P., Wei Y.J., Zhang W.F., Zhang Y., Liu S.K., and Fang J.X.J., 2015, Draft genome sequence of Bacillus thuringiensis strain S2685-1, a novel strain with high larvacidal toxicity against Helicoverpa zea, Bt Research, Vol.6, No.9 1-4 (doi: 10.5376/bt.2015.06.0009)
Here, we report the draft genome sequence of Bt Strain S2685-1. It consists of 262 scaffolds with a GC content of 34.89% and a total length of 5,685,503 bp. The assemble analysis shows that there are four replicons in S2685-1, including a circular chromosome of 5,287,484 bp with 6326 ORFs and 5429 CDSs and three circular plasmids, which are named S2685-1P01 (151,717 bp), S2685-1P02 (196,775 bp) and S2685-1P03 (49,527 bp), respectively. The average GC content of the chromosome sequence is 35.08%, while that of the plasmid sequences are 31.86%, 32.23% and 34.89%, respectively. Two crystal genes have been predicted from this strain but they share 100% sequence identity to cry1Ac in HD73. It also had been identified with high larvacidal toxicity against Helicoverpa zea in S2685-1.
Bacillus thuringiensis; S2685-1; Larvacidal Toxicity; Helicoverpa zea