Research Report

Draft Genome Sequence of Bacillus thuringiensis Strain S2685-1, a Novel Strain with High Larvacidal Toxicity against Helicoverpa zea  

Zhou  Y.1,2,* , Wu  Z.Q.3,* , Liu  P.P.3 , Wei  Y.J.4 , Zhang  W.F.5 , Zhang  Y.4 , Liu  S.K.3 , Fang  J.X.J.1,2,3,6
1 Hainan Institute of Tropical Agricultural Resources (HITAR), Sanya, China
2 College of Life and Technology Science, Guangxi University, Nanning, Guangxi, China
3 Alkali Soil Natural Environmental Science Center(ASNESC), Northeast Forestry University, Harbin, 150040, China
4 College of Bioinformatics Science and Technology, Harbin Medical University, Harbin, China
5 College of Life Sciences, Hainan Normal University, Haikou, China
6 The HITAR Institute Canada Inc. British Columbia, Canada * These authors contributed equally.
Author    Correspondence author
Bt Research, 2015, Vol. 6, No. 9   doi: 10.5376/bt.2015.06.0009
Received: 30 Oct., 2015    Accepted: 20 Nov., 2015    Published: 16 Dec., 2015
© 2015 BioPublisher Publishing Platform
This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Zhou Y., Wu Z.Q., Liu P.P., Wei Y.J., Zhang W.F., Zhang Y., Liu S.K., and Fang J.X.J., 2015, Draft genome sequence of Bacillus thuringiensis strain S2685-1, a novel strain with high larvacidal toxicity against Helicoverpa zea, Bt Research, Vol.6, No.9 1-4 (doi: 10.5376/bt.2015.06.0009)

Abstract

Here, we report the draft genome sequence of Bt Strain S2685-1. It consists of 262 scaffolds with a GC content of 34.89% and a total length of 5,685,503 bp. The assemble analysis shows that there are four replicons in S2685-1, including a circular chromosome of 5,287,484 bp with 6326 ORFs and 5429 CDSs and three circular plasmids, which are named S2685-1P01 (151,717 bp), S2685-1P02 (196,775 bp) and S2685-1P03 (49,527 bp), respectively. The average GC content of the chromosome sequence is 35.08%, while that of the plasmid sequences are 31.86%, 32.23% and 34.89%, respectively. Two crystal genes have been predicted from this strain but they share 100% sequence identity to cry1Ac in HD73. It also had been identified with high larvacidal toxicity against Helicoverpa zea in S2685-1.

Keywords
Bacillus thuringiensis; S2685-1; Larvacidal Toxicity; Helicoverpa zea
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