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Bt S2685-1, A Bacillus thuringiensis Strain with Significantly Larvacidal Toxicity against Corn Earworm (Helicoverpa zea)  

Xuanjun Fang , Yan Zhou , Wenfei Zhang , Liu Xie , Jintian Zhu
Hainan Institute of Tropical Agricultural Resources, Sanya, 572025, Hainan, China
Author    Correspondence author
Bt Research, 2015, Vol. 6, No. 10   doi: 10.5376/bt.2016.06.0010
Received: 30 Oct., 2015    Accepted: 24 Nov., 2015    Published: 26 Dec., 2015
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This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Fang X.J., Zhou Y., Zhang W.F., Xie L., and Zhu J.T., 2015, Bt S2685-1, A Bacillus thuringiensis strain with significantly larvacidal toxicity against corn earworm (Helicoverpa zea), Bt Research, Vol.6, No.10, 1-2 (doi: 10.5376/bt.2015.06.0010)

Abstract

Bt Strain S2685-1 was identified by Dr. X. J. Fang’s group from Hainan Institute of Tropical Agricultural Resources (HITAR) in 2009 through 2014. The isolate (isolate No: 20070720S2685) was discovered from soil sample that were collected from Heilongjiang Liangshui Natural Reserves of China. Bt S2685-1 produces dominant 130 kDa crystalline protein with significant larvicidal effects on corn earworm (Helicoverpa zea). Scanning electron microscope observation showed that the strain generates high-yielding bipyramidal crystals during sporulation. SDS-PAGE analysis showed that the protein profile mainly contains six proteins with the molecular weight about 130 kDa, 80 kDa, 75 kDa, 45 kDa, 37 kDa, 25 kDa. The mega-plasmid profile of the Bt S2685-1 showed that it had three mega-plasmid (70 kb, 97 kb, 200 kb). The genome sequencing predicts that it contains cry1A-like gene. Interestingly, the bioassays were carried out on mosquitoes in Diptera and corn earworm (Helicoverpa zea) in Lepidoptera, the results exhibit that the strain Bt S2685-1 is significantly toxic to corn earworm (Helicoverpa zea) larva, but not in mosquitoes. Up to now, we haven’t got the predicted expression products, so, what we need to do next is cloning and expressing the toxic genes, and further focus on other Lepidoptera or Coleoptera insects in agriculture. 

Keywords
Bacillus thuringiensis; Bt isolate 20070720S2685; Bt Strain 2685-1; Cry1A-like gene; Corn earworm (Helicoverpa zea)
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. Bacillus thuringiensis
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