Research Report

Identification and Characterization of Bacillus thuringiensis Strain BRC-ZLL13 with High Toxic Activity Against Mosquitoes  

Liu Zhaoxia1 , Li Mingwei1 , Wu Songqing1,2 , Ye Wenhui1 , Yu Xiongze1 , Lin Qiuqiu1 , Guan Xiong1 , Zhang Lingling1
1 Key Laboratoryof Biopesticide and Chemical Biology, Ministry of Education, Fujian Agriculture and Forestry University, Fuzhou, 350002
2 Collegeof Forestry, Fujian Agriculture and Forestry University, Fuzhou, 350002
Author    Correspondence author
Bt Research, 2014, Vol. 5, No. 5   doi: 10.5376/bt.2014.05.0005
Received: 01 Sep., 2014    Accepted: 01 Oct., 2014    Published: 01 Nov., 2014
© 2014 BioPublisher Publishing Platform
This article was first published in Genomics and Applied Biology in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Liu Z.X., Li M.W., Wu S.Q., Ye W.H., Yu X.Z., Lin Q.Q., Guan X., and Zhang L.L., 2014, Identification and Characterization of Bacillus thuringiensis Strain BRC-ZLL13 with High Toxic Activity Against Mosquitoes, Bt Research, 5(5): 1-8 (doi: 10.5376/bt.2014.05.0005)

Abstract

Bacillus thuringiensis strain BRC-ZLL13 was isolated from leaves of Magnolia aenudate. It was characterized based on its insecticidal activity against Aedes aegypti, Aedes albopictus, and Culex quinquefasciatus larvae, flagellar antigen serology, biochemical reactions, antibiotic sensitivity, plasmid profile and crystal protein profile as well as PCR analysis using novel general and specific primers for cry and cyt genes analysis. It showed more than twofold higher mosquitocidal activity against A. aegypti, A. albopictus and C. quinquefasciatus larvae than B. thuringiensis subsp. israelensis (IPS82) and displayed high similarities with the IPS82 with regard to protein and the presence of mosquitocidal genes, but had different plasmid profiles and higher growth rate. These results suggested that the loss of plasmid might be contribute to high growth rate of this strain, and indicated a potential for developing this strain for application in the fields of public hygiene and biological control.

Keywords
Bacillus thuringiensis; Mosquitocidal activity; Biological property
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