Draft Genome Sequence of Bacillus thuringiensis Strain S3076-1
1 Alkali Soil Natural Environmental Source Center, Northeast Forestry University, Harbin, 150040
2 Hainan Institute of Tropical Agricultural Resources, Sanya, 572025
3 Institute of Life Science, Jiyang College of Zhejiang A&F University, Zhuji, 311800
4 Cuixi Academy of Biotechnology, Zhuji, 311800
5 College of Life Sciences and Technology, Guangxi University, Nanning, 530005
6 College of Bioinformatics Science and Technology, Harbin Medical University, Harbin, 150040
Bt Research, 2016, Vol. 7, No. 1 doi: 10.5376/bt.2016.07.0001
Received: 16 Nov., 2016 Accepted: 20 Nov., 2016 Published: 22 Nov., 2016
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Preferred citation for this article:
Wu et al., 2016, Draft genome sequence of Bacillus thuringiensis strain S3076-1, Bt Research, Vol.7, No.1 1-7 (doi: 10.5376/bt.2016.07.0001)
Abstract Bacillus thuringiensis S3076-1 can produce some parasporal crystal with a variety of forms, which may have insecticidal activity to Lepidoptera insects. In this study, the de novo whole genome sequence of Bt S3076-1 was carried out by employing Illumina HiSeq 2000 and generated 1.42 Gb raw data. Through the original reads data quality control, filtering and SOAPdenovo short sequence assembly to get a total of 231 scaffolds, the genome size was 6.42 Mb, N50 length was 97325 bp, GC content of 34.81%. The genomic information of 14 Bt strains in NCBI database were selected as the reference, and the genomic sequences of Scaffolds were assembled to obtain the 3 replicators. Among them, the size of the genome was 5.72 Mb, the content of GC was about 35.00%, and the length of the genomic sequences of the four plasmids were 181 kb and 518 kb, and the GC contents were 33.10% and 33.13%, respectively. Based on the genome sequence and annotation information, the genome of the visual sketch was created by GCviewer server. The whole genome sequencing and construction of Bt S3076-1 provide the basis for the functional study of the strain, and contribute to the identification of Bt S3076-1 insecticidal toxin protein.
Bacillus thuringiensis; Bt S3076-1; Whole genome sequencing; Genome assembling; Draft genome