AtGS1.2 Gene May Enhance Salt Stress Tolerance in Seed Germination and Overexpression of Arabidopsis thaliana
1. Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field (SAVER), Ministry of Education, Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, Harbin, 150040
2. Asian Natural Environmental Science Center (ANESC), The University of Tokyo, Nishi-tokyo-shi, Tokyo, Japan
Cell Biology and Biophysics, 2015, Vol. 4, No. 2 doi: 10.5376/cbb.2015.04.0002
Received: 08 Jun., 2015 Accepted: 22 Jun., 2015 Published: 29 Jun., 2015
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Preferred citation for this article:
Lin Xu, Daisuke Tsugama, Tetsuo Takano, Yuanyuan Bu and Shenkui Liu, 2015, AtGS1.2 Gene May Enhance Salt Stress Tolerance in Seed Germination and Overexpression of Arabidopsis thaliana, Cell Biology and Biophysics, 4(2): 1-6 (doi: 10.5376/cbb.2015.04.0002)
Glutamine synthetase (GS) is the key enzyme of ammonium assimilation in plants. In this research, we focus on GS1.2 (AtGS1.2) in Arabidopsis thaliana, and study the function of ammonium assimilation in relieving salt stress. Semi-quantitative PCR was employed to investigate the expression of AtGS1.2 in different time. The expression level of AtGS1.2 under the salt-treatment during seed germination stage was analyzed by northern blot method. The result indicated that the expression of AtGS1.2 was induced by 100 mM NaCl treatment during seed germination, comparing with control. We also obtained the over-expression lines of AtGS1.2 to analyze the functions at salt stress. According to the results, we can infer that AtGS1.2 in Arabidopsis plays an important role in the defense of salt stress during seed germination and seeding stages.
Arabidopsis; Glutamine synthetase; NaCl stress; Seed germination
Cell Biology and Biophysics
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