AtCAX1 N-terminal can improve Ca2+ and Ba2+ tolerance in yeast  

Hongjun Guo1 , Daisuke Tsugama2 , Tetsuo Takano2 , Yuanyuan Bu1 , Shenkui Liu1
1. Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field (SAVER), Ministry of Education, Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, Harbin, 150040
2. Asian Natural Environmental Science Center (ANESC), The University of Tokyo, Nishi-tokyo-shi, Tokyo, Japan
Author    Correspondence author
Cell Biology and Biophysics, 2015, Vol. 4, No. 3   doi: 10.5376/cbb.2015.04.0003
Received: 08 Jun., 2015    Accepted: 23 Jun., 2015    Published: 29 Jun., 2015
© 2015 BioPublisher Publishing Platform
This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Hongjun Guo, Daisuke Tsugama, Tetsuo Takano, Yuanyuan Bu and Shenkui Liu, 2015, AtCAX1 N-terminal may enhance Ca2+ and Ba2+ tolerance in yeast, Cell Biology and Biophysics, 4(3): 1-4 (doi: 10.5376/cbb.2015.04.0003)

Abstract

AtCAX1 is a member of the Ca2+/H+ antiporter family. The N-terminally truncated AtCAX1 (ΔN108AtCAX1) and the C-terminally segment truncated AtCAX1 (ΔC9AtCAX1, ΔC36AtCAX1, and ΔC144AtCAX) and full-length AtCAX1 were created and analyzed their growth in the presence of Ba2+ and Ca2+. The ΔN108AtCAX1 grew well in the presence of 150 mM Ca2+ and 15mM Ba2+. This result indicates that ΔN108AtCAX1 can improve the resistance to Ca2+ and Ba2+. And by atomic absorption spectrophotometer to measure the Ba2+ content of whole yeast cells, the results showed that the Ba2+ content in yeast cell expressing ΔN108AtCAX1 was lower than other yeast transformants. The studies indicate that AtCAX1 may transport Ca2+ and Ba2+.

Keywords
AtCAX1; Ca2+ and Ba2+ tolerance; Auto-inhibitory domain
[Full-Text PDF] [Full-Text HTML]
Cell Biology and Biophysics
• Volume 4
View Options
. PDF(550KB)
. HTML
Associated material
. Readers' comments
Other articles by authors
. Hongjun Guo
. Daisuke Tsugama
. Tetsuo Takano
. Yuanyuan Bu
. Shenkui Liu
Related articles
. AtCAX1
. Ca 2+ and Ba 2+ tolerance
. Auto-inhibitory domain
Tools
. Email to a friend
. Post a comment