Proteomic Analysis of Chloris virgata Leaves under Carbonate Stress
1 Alkali Soil Natural Environmental Science Center (ASNESC), Stress Molecular Biology Laboratory, Northeast Forestry University, Harbin, 150040, P.R. China;
2 Asian Natural Environment Science Center (ANESC), the University of Tokyo, Midori Cho 1-1-1, Nishitokyo City, Tokyo 188-0002, Japan
Cell Biology and Biophysics, 2013, Vol. 2, No. 1 doi: 10.5376/cbb.2013.02.0001
Received: 10 Mar., 2013 Accepted: 15 Apr., 2013 Published: 30 May, 2013
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Preferred citation for this article:
Luo et al., 2013, Proteomic Analysis of Chloris virgata Leaves under Carbonate Stress, Cell Biology and Biophysics, Vol.2, No.1 1-11 (doi: 10.5376/cbb.2013.02.0001)
Carbonate stress, mainly due to high content of sodium carbonate and sodium bicarbonate in the soil, which are more severe to crop plants than sodium chloride stress, is one of the major problems in northern China. Chloris virgata (Swartz) is a carbonate tolerant plant that can grow around the alkali spots naturally. Here we report a systematic proteomic approach to identify the carbonate stress responsive proteins of Chloris virgata leaves under carbonate stress. 2 week-old seedlings were treated with 80 mM NaHCO3 for 24, 48 and 72 h, with distilled water as the controlled variable. Leaf samples were analyzed using Two-dimensional electrophoresis (2-DE). More than 500 protein spots that reproduced were detected, among which 56 spots showed significant response to sodium bicarbonate stress compared to the control. 23 protein spots were identified by MALDI-TOF MS and/or TOF-TOF MS analysis, classified to six functional categories including photosynthesis, metabolism, protease, transcriptional and translational factors, as well as other proteins and unknown proteins. The mRNA transcription levels of some protein spots corresponding to genes respond to NaHCO3 stress were examined. Morphology of Chloris virgata seedlings were also analyzed compared to rice plant (Oryza Sativa L. cv Nipponbare), which was classified as moderate salt tolerance among eight rice cultivars tested for their relative sensitivity to salt stress. This study analyzed the halophytes leaves’ protein response to carbonate stress by proteomic analysis, which will give a new insight into alkalinity stress response in plants.
Carbonate stress; Chloris virgata; MALDI-TOF MS; Two dimensional electrophoresis