The Effect of Arbuscular Mycorrhiza Fungi on Cotton Growth and Yield under Salinated Soil Condition  

Botir Khaitov , Shukhrat Teshaev
Faculty of Agronomy, Tashkent State Agrarian University, 100140, Tashkent, Uzbekistan
Author    Correspondence author
Cotton Genomics and Genetics, 2015, Vol. 6, No. 3   doi: 10.5376/cgg.2015.06.0003
Received: 21 Apr., 2015    Accepted: 08 Jun., 2015    Published: 17 Jun., 2015
© 2015 BioPublisher Publishing Platform
This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Khaitov B., 2015, Genetic Diversity Analysis for Earliness, Fiber Quality and Cotton Leaf Curl Virus in Gossypium hirsutum L. Accessions, Cotton Genomics and Genetics, Vol.6, No.3 1-7 (doi: 10.5376/cgg.2015.06.0003)


The aim of this study was to investigate the response of two cotton AN-Bayaut-2 and S-6524 (Gossipium hirsutum L.) varieties to Arbuscular Micorrhiza Fungi (AMF) inoculation under salinated soil condition. The inoculation significantly increased N, P uptake and plant growth of both cotton varieties but micorrhization rate were considerable higher in AN-Bayaut-2 compare to S-6524 variety, 36,7% and 19.1% respectively. Micorrhization rate of root had a significant impact on plant growth and development during vegetation period. AMF formed a symbiotic relationship with cotton root in salinated soil enhanced a nutrient and water uptake of plant and growth of the plant as well as cotton yield.


Arbuscular Micorrhiza fungi (AMF); Cotton; Plant growth; Nutrient uptake

Cotton is one of the major fibre crop of worldwide as well in Uzbekistan. Although it is classified as salt tolerant plant (Maas and Hoffman, 1977) but it is sensitive in salination at germination stage (Ahmad et al, 2002). Salinity induced phytotoxicity including necrosis of apices and leaf margin, stunted growth, and leaf chlorosis (Ahmad et al., 2002). Increasing rate of saline water in agricultural fields leads to a major threat to plant production and hence retards the growth and development of plants (Shokri and Maadi, 2009) by affecting various metabolic processes.

Soil salinity is becoming more serious problem each passed year in agricultural production of Uzbekistan. Moreover, drying up of Aral Sea is exacerbating current already deteriorated soil as well ecological condition. Although, cotton production significantly decreased in during last decades, it is still counts as the main cropcovering 1.3 mln ha of arable field almost same amount of area as wheat.

Plants grown in the field are surrounded by various microorganisms such as bacteria and fungi that help and improve the plant growth and yield under various stress conditions (Egamberdieva et al., 2007). There have been many reports describing potential benefit of AMF on nutrient uptake of cotton in soil salinity condition, thereby increasing growth and development of cotton plant in harsh environmental condition (Linderman, 1992; Egamberdieva et al., 2007). However, not all plant species form AM associations, and not all AM plant species show clear nutritional benefits from colonization by mycorrhizal fungi under all growth conditions (Connor et al., 2002). Since the mycorrhizosphere is greatly influenced by the host plant genotype (Linderman, 1992), to date there is no any study describing AMF symbioses with new cotton varieties grown in harsh soil salinated condition of Uzbekistan. The objectives of our research were to study the response of two different cotton varieties (Gossipium hirsutum L.) grown on salinated serozem soil with treatment AMF under arid climatic conditions.

Material and Methods
The field experiment was established in three replications on salinated serozem soil of Oq Oltin district Sirdarya region.  Electrical conductivity (EC) values of saline soil were 6.2 dS/m. According to the FAO classification, the soils are calcareous calcisol with low nutrient contents. Cotton seed Gossipium hirsutum L. variety AN-Bayaut-2 and S-6524 were obtained from Seed laboratory of Tashkent State Agrarian University.

The cotton seeds were soaked during 6 hours and inoculated AMF Glomus mosseae hyphae/spores before planting them on experimental plot. For the inoculation with mycorrhiza ~50 g of Glomus mosseae inoculum (BEG 12; International Bank of Glomeromycota; was added to 1 kg cotton seeds and planted.

The criteria for growth promotion were studied in root and shoot dry matter, N and P content of plants were analysed. After 4~8 weeks, shoots and roots were separated and dried. The total N content in plant tissues was determined following the semi-micro Kjedahl procedure. The P content was determined spectrophotometrically using classic methods of P measurement. The roots were carefully washed free of soil and the mycorrhizal colonisation of the roots was assessed using the methods of Vierheiling et al. (1998). Plant samples from field were also collected for mycorrhizal assessment and to compare with root box data.  At the end of growing season the two center rows of each plot were harvested, weighed and ginned and lint yield and percent lint were determined. Statistical analysis

Data were tested for statistical significance using the analysis of variance package included in Microsoft Excel 98 and comparison was done using a Student’s t-test. Mean comparisons were conducted using a least significant difference (LSD) test (P=0,05). Standard error and a LSD result were calculated.

Results and Discussion
Salinity decreases shoot/root ratio of plant because shoots are sensitive than roots to salts stress (Nazarov, 2007). In another study Lin et al. (1997) observed that increasing salinity (NaCl) decreased the protein content in the seedlings and increased the enzyme activity in both salt tolerant and salt sensitive cotton cultivars. Growth and biomass inhibition of cotton varieties under salt stress are caused due to high accumulation of NaCl salt.

Cotton is a mycotrophic plant in which growth and nutrient uptake is usually increased by AM colonisation. It is known that most terrestrial plants live in symbiosis with AMF (Hoffmann, 2009), and in most cases AMF symbiosis improves the nutritional status of the host plant and thus, its overall fitness (Read, 1998). However, it is uncertain whether slow AM colonisation in the cotton growth disorder is a symptom or the cause of reduced plant growth (Nehl et al. 1998). Some authors declared that the highest growth stimulation of plant by beneficial bacteria and fungi occurred when plant have encountered stressful conditions, whereas non-treated plants by comparison performed poorly under such conditions (Egamberdieva, 2013; Nehlet al. 1998).

In our experiments inoculation of cotton varieties with AMF caused increase in plant height, number of bolls per plant, and overall cotton yield compared uninoculated plants in saline soil condition.

In AN-Bayaut-2 variety, inoculation with AMF resulted in increased shoot and root development, the fresh and the dry weight of the shoot and root (Table 1), the number of capsules, and the shoot P and N content (Table 2). Also, in S-6524 variety were observed the significant increase of above indicated parameters of growth but slightly lower than AN-Bayaut-2 (Table 3).  

Table 1 The effect of AMF on plant growth of cotton at soil salinity conditions
Note: 1 g/plant, plants grown for 4 week; 2 g/plant, plants grown for 8 week



Table 2 Mycorrhizal colonization and P and N content of plant


Table 3 The effect of inoculation with AMF on cotton yield, centner /ha

At 8 weeks, root and shoot dry weights were significantly greater at the AMF symbioses cotton plants (Table 1). Also, significant differences determined between cotton varieties in root and shoot weight at this stage. Also, shoot and root fresh weights (Table 1) were significantly increased by the mycorrhizal associations, although no major effects on shoot:root ratios were apparent.

In AN-Bayaut-2 variety, inoculation with AMF resulted in increased shoot and root development, the fresh and the dry weight of the shoot and root (Table 1), the number of capsules, and the shoot P and N content (Table 2). Also,

Plants when colonized by AM fungi show high degree of protection by accumulating more and more solute as it has been indicated in many plants (Evelin et al., 2009). Similar results were observed by Patale and Shinde (2012) where they found salinity affected the plant growth at higher salt concentrations and inoculated plants were healthier at low to medium salt concentrations while could obtained necessary nutrients from the soil.

Accordingly, to combat the poor supply of nutrients from the soil, mycorrhizal fungi help their host plant to restore the uptake of mineral nutrients and hence plant growth (Sharifiet et al., 2007). AM fungal symbiosis plays a key role in supplying the poor mobility nutrients like phosphorus to the host plant by the roots and hence suppress the negative effects of salt (Feng et al., 2002).

The root and shoot dry weight showed substantial differences with approximately 1.2-1.3 times greater than non-inoculated one in both cotton varieties. Mycorrhiza inoculated plants in medium salinated soil of Sirdarya region had higher root mass and colonization compared to non-inoculated cotton plants. The percentage of root colonization in AN-Bayaut-2 was almost two times more than S-6524; 36.7 % and 19.1 respectively (Table 2).

No any AMF root colonization was found in root system of control plants, we suppose there were no any ubiquitous AMF on the soil. According observations Linderman (1992), the fungi and hosts of the various types of mycorrhizae are so different, it is important to recognise that aspects of the symbiosis may also be very different.

P and root N content of plants biomass (Table 2) were considerably increased by the mycorrhizal associ- ations, the highest P and N recorded on AN- Bayaut-2 variety. Similar results were observed by Ortas (2012) where he revealed mycorrhiza inoculated plants had a higher nutrient content than non-inoculated plants and inoculation effectiveness was higher under stress condition than normal one.

As AN-Bayaut-2 is responsive variety tended to have higher levels of infection and S-6524 is less responsive variety tended to have lower levels of AMF infection in salinated soil condition. AM fungi play a key role in alleviating the toxicity induced by salt stress, thus normalizing the uptake mechanism in plants by supplying the essential nutrients (Carretero et al., 2008; Porcel et al., 2012).

Microorganisms such as bacteria and fungi increase plant growth and yield under adverse environmental conditions as they have the tendency to resist the damage and hence develop resistance against harmful effects of salinity stress (Asiya et al., 2014).

The response within the varieties to AMF inoculation significantly differed almost in all growth parameters; AN-Bayaut-2 variety showed greater response from beginning of growth period; root and shoot dry weight and P content in shoot was higher than the S-6524 variety. And at the end of growing season cotton yield was significantly higher in AN-Bayaut-2 cotton variety comparing to S-6524. Also, inoculation with AMF resulted in a 21% and 17% increase in the weight of bolls plant−1 and cotton fibre qualities, respectively, as compared with the uninoculated control (data not shown).

According to (Gargand Baher, 2013) arbuscular mycorrhizal (AM) fungi are considered to be bioameliorators of salinity stress due to their capability to live in contaminated soils and their role in modulation of biochemical processes. The role of the AM fungi as P suppliers to the plant appears to be of great relevance, and AMfungi may solubilize P from surrounding areas and make it available to roots (Behl et al., 2003). Thus, in both varieties AN-Bayaut-2 and S-6524, inoculation with AMF produced the greatest effect on both plant growth and nutrient uptake, together with a noticeable increase in mycorrhizal root colonization.

We observed that cotton growth and development stimulation by symbioses AMF was higher in soil salinated condition compared to without symbioses. It can be concluded that AMF is important under soil salinity stress condition to help the plant to get more nutrients from the soil and the importance of AMF towards cotton varieties is variable. We revealed there was a significant relationship between AMF colonization among the cotton varieties. In conclusion, the results of the present study suggest that the inoculation of AM fungi in cotton varieties in soil salinated condition improved the plant growth, development and yield, thus suggesting that it can provide an alternative to chemical fertilizers.

This research was funded by the OEAD and CASIA post doctoral fellowships.

Carlos Luis Carretero, Cantos M., Garcia J.L., Azcon R., and TroncosoA. 2008, Arbuscular-mycorrhizal contributes to alleviation of salt damage in cassava clones, Plant Nutr 31:959–971  

Daniella Hoffmann, Vierheilig H., Riegler P., and Schausberger P., 2009, Arbuscular mycorrhizal symbiosis increases host plant acceptance and population growth rates of the two-spotted spider mite Tetranychus urticae, Oecologia 158:663–671  

David B. Nehl, Allen S.J., and Brown J.F., 1998, Slow arbuscularmycorrhizal colonisation of field-grown cotton caused by environmental conditions in the soil, Mycorrhiza 8:159–167  

David J. Read, 1998, Mycorrhiza - the state of the art. In: Varma A., Hock B. (eds) Mycorrhiza, Springer, Berlin, 3–34  

Dilfuza Egamberdieva, 2013, Response of Maize to Bacterial Inoculants under Different Soil Conditions, Turkey International Plant Nutrition Colloquium. XVII: 583-585

Dilfuza Egamberdiyeva, Gafurova L., and Islam R., 2007, Salinity effects on irrigated soil chemical and biological properties in the Syr-Darya basin of Uzbekistan, in: R. Lal et al. Climate Change and Terrestrial C Sequestration in Central Asia, Taylor and Francis, New York, 147-162.

Eugene V. Maas, and Hoffman G.J., 1977, Crop salt tolerance-Current assessment, Irrig. Drain. Div. Am. Soc. Sic. Eng., 103: 115-134

Gu Feng, Zhang F.S., Li X.l., Tian C.Y., Tang C., and Rengel Z., 2002, Improved tolerance of maize plants to salt stress by Arbuscular mycorrhiza is related to higher accumulation of soluble sugars in roots, Mycorrhiza 12:185–190  

Gwen Smith, and Roncadori R.W., 1986, Responses of three vesicular arbuscular mycorrhizal fungi at four soil temperatures and their effects on cotton growth, New Phytol. 104: 89–95  

Hazin Asiya, Egamberdieva D., Elsayed F.A., Abeer H., Ashwani K., and Parvaiz A., 2014, Salinity stress and arbuscular mycorrhizal symbiosis in plants, Review. 01/139-159

Heikham Evelin, Kapoor R., and Giri B., 2009, Arbuscular mycorrhizal fungi in alleviation of salt stress: a review. Ann Bot 104:1263–1280  

Horst Vierheilig, Coughlan A.P., Wyss U., and Piche Y., 1998, Ink and vinegar, a simple staining technique for arbuscular-mycorrhizal fungi, Applied and Environmental Microbiology 64(12), 5004-5007

Hsin Lin, Salus S.S., and Shumaker K.S., 1997, Salt sensitivity and the activities of the H+ ATP ases in cotton seedlings, Crop Sci., 37: 190-197  

Ibrahim Ortas, 2012,The effect of mycorrhizal fungal inoculation on plant yield, nutrient uptake and inoculation effectiveness under long-term field conditions, Field crops research 125: 35-48  

Jian-Di Lin, Zhu Z.Y., and Fan B.X., 1995, Physiological reaction of cotton varieties under different levels of salt stress, China Cotton, 22: 16-17

Leide E.O., Nogales R., Lips S.H., 1991, Effect of Salinity on cotton plant growth under nitrate or ammonium nutrition at different calcium levels, Field Crops Res., 26: 35-44  

Mozafar Sharifi, Ghorbanli M., and Ebrahimzadeh H., 2007, Improved growth of salinity-stressed soybean after inoculation with salt pre-treated mycorrhizal fungi, Plant Physiol 164:1144–1151  

Neera Garg, Baher N., 2013, Role of arbuscular mycorrhizal symbiosis in proline biosynthesis and metabolism of Cicer arietinum L. (Chickpea) Genotypes Under Salt Stress, Plant Growth Regul 32:767–778  

Patale S.W., and Shinde B.P., 2012, Effect of Salinity Stress on Growth Performance of Bt-Cotton Inoculated with AM Fungi, Plant Sciences 25:135-139

Patrick J. Connor O'., Smith S.E., and Smith F.A., 2002, Arbuscular mycorrhizas influence plant diversity and community structure in a semi arid herb land, New Phytologist 154.1: 209-218  

Rinat Nazarov, 2007, Efficient irrigation and fertilization of cotton, Agriculture of Uzbekistan 4: 33

Rishi K. Behl, Sharma H., Kumar V., and Narula N., 2003, Interactions amongst mycorrhiza, Azotobacter chroococcum, and root characteristics of wheat varieties, Agronomy and Crop Science 189:151–155  

Robert G. Linderman, 1992, Vesicular-arbuscular mycorrhizae and soil microbial interactions, In: Bethlenfalvay G.J., Linderman R.G. (eds) Mycorrhizae in sustainable agriculture, American Society of Agronomy, Madison, Wis. 45–70

Rosa Porcel, Aroca R., and Ruíz-Lozano J.M., 2012, Salinity stress alleviation using Arbuscular mycorrhizal fungi, A review. Agron Sustain Dev 32:181–200  

Saeed Shokri, and Maadi B., 2009, Effects of Arbuscular mycorrhizal fungus on the mineral nutrition and yield of Trifolium alexandrinum plants under salinity stress, Agronomy 8(2):79–83  

Saghir Ahmad, Khan N.I., Iqbal M.Z., Hussain A., and Hassan M., 2002, Salt tolerance of cotton (Gossipium hirsutum L.)., Asian Journal of Plant Sciences 6: 715-719

Shukhrat Teshaev, and Kodirxujaeva M., 2003, Application of mineral fertilisers to cotton variety S-6524 and efficiency of defoliation, News Agrarian sciences. 1(11):51-55 

Cotton Genomics and Genetics
• Volume 6
View Options
. PDF(0KB)
. Online fPDF
Associated material
. Readers' comments
Other articles by authors
. Botir Khaitov
. Shukhrat Teshaev
Related articles
. Arbuscular Micorrhiza fungi (AMF)
. Cotton
. Plant growth
. Nutrient uptake
. Email to a friend
. Post a comment