Agrobacterium-mediated Transformation of Cotton Shoot Apex with SNC1 Gene and Resistance to Cotton Fusarium Wilt in T1 Generation
Institute of Nuclear and Biological Technology, Xinjiang Academy of Agricultural Sciences, Urumqi, 830091
Cotton Genomics and Genetics, 2012, Vol. 3, No. 1 doi: 10.5376/cgg.2012.03.0001
Received: 18 Jan., 2012 Accepted: 02 Jul., 2012 Published: 09 Jul., 2012
© 2012 BioPublisher Publishing Platform
This article was first published in Molecular Plant Breeding (2010, Vol.8, No.2, 252-258) in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:
Lei et al., 2011, Agrobacterium-mediated Transformation of Cotton Shoot Apex with SNC1 Gene and Resistance to Cotton Fusarium Wilt in T1 Generation, Cotton Genomics and Genetics, Vol.3 No.1 1-7 (doi:10.5376/cgg.2012.03.0001)
Using shoot apexes from the upland cotton varieties, named Zhong 35 and Junmian No.1 as explant, the SNC1, suppressor of npr1-1constitutive cloned from Arabidopsis thaliana, was introduced into cotton genome by Agrobacterium-mediated transformation with the optimized transformation conditions including culture stage of the explant, infection time and co-culture time. The results showed that the transformation procedure for high transformation rate was as follows, in vitro cuture the explants one day, infecting 20 min and co-culturing two days. PCR and RT-PCR identification of T0 and T1 regeneration plants revealed that the SNC1 gene was successfully integrated into cotton genome and expressed in the transgenic plant. Furthermore, comparing with non-transgenic plants, the T1 generation transgenic plants acquired the significant resistance to the inoculation of with the Fusarium oxysporum f.sp.Vasinfectum by the validation of the root-dipping method, which suggested that the SNC1 should facilitate the resistant to cotton Fusarium wilt.
Upland cotton;SNC1; Agrobacterium-mediated transformation; Fusarium wilt