Identification of A Novel phaC1 Gene from Pseudomonas putida KT2442  

Jamshid Raheb , Saeed Saadat , Fatemeh  Nazari
National Institute of Genetic Engineering and Biotechnology, Tehran, Iran
Author    Correspondence author
Computational Molecular Biology, 2013, Vol. 3, No. 3   doi: 10.5376/cmb.2013.03.0003
Received: 06 Nov., 2013    Accepted: 05 Dec., 2013    Published: 26 Dec., 2013
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This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Raheb et al., 2013, Identification of a Novel phaC1 Gene from Native Pseudomonas putida KT2442 as a Key Gene for PHA Biosynthesis, Computational Molecular Biology, Vol.3, No.3 16-23 (doi: 10.5376/cmb.2013.03.0003)

Abstract

Economic activities have increased over the past century which leads to inter-related problems that require immediate consideration. Today, due to the excessive consumption of plastics, development of bio-degradable polymers is an important element for the economic progress. In addition nano-beads application is developed that are of interest for industrial and biomedical applications. These biodegradable biopolymers, polyhydroxyalkanoates (PHAs) are a family of polyhydroxyesters of 3, 4, 5 and 6 hydroxyalkanoic acids produced by a wide range of bacteria as granules in the cytoplasm of the cells under growth limiting conditions with carbon excess. These carbon and energy storage polymers are bio-degradable and insoluble in water, show thermoplastic properties and they can be produced from renewable carbon sources. In this study, PHA produced by native Pseudomonas putida strain was investigated by Methanolysis and FT-IR methods. The results showed that the bacterium is capable of producing PHA. Then, using appropriate primers and the polymerase chain reaction, PHA synthase gene of the organism was amplified, sequenced and compared to NCBI registered sequences.

Keywords
Nano-beads; Polyhydroxyalkanoates; Pseudomonas putida
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