Research Report

In Vitro Evaluation of Different Chemicals against Rhizoctonia solani by Poisoned Food Technique  

Manju Adhikari1 , Neeta Kharel1 , Lila Gaire1 , Riturani Poudel1 , Sundar Man Shrestha2 , Shankar Prasad Gaire2 , Basistha Acharya2
1 Institute of Agriculture and Animal Science, Rampur, Chitwan, Nepal
2 Agriculture and Forestry University, Rampur, Chitwan, Nepal
Author    Correspondence author
Field Crop, 2018, Vol. 1, No. 2   
Received: 08 Jun., 2018    Accepted: 29 Jun., 2018    Published: 20 Jul., 2018
© 2018 BioPublisher Publishing Platform
This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract

A lab experiment was conducted to evaluate different fungicides against Rhizoctonia solani by poisoned food technique in completely randomized design with 4 replications at Plant Pathology lab, Institute of Agriculture and Animal Science, Rampur in 2072 BS. The sclerotia were collected from diseased maize of National Maize Research Program, Chitwan and were grown on PDA slant tubes to prepare pure culture. The fungicides namely; Saff (Carbendazim 12% + Mancozeb 63%), Allcop (Copper oxychloride 50 w/w), Protector (Chlorothalonil 75% wp) and Vitavax power (Carboxin 37.5% + Thiram 37.5%) were added in the PDA medium @ 10 ppm, 50 ppm, and 100 ppm and mycelia bits of 10 mm diameter were inoculated at the center. The measurement of mycelia radial growth was taken of the petri plates at 48, 72, 96 and 120 hours after inoculation. All three concentrations of four fungicides significantly inhibited mycelial growth as compared to control. However Vitavax was found more effective in inhibiting mycelial radial growth of the pathogen at lower concentration (10 ppm) and Copper oxychloride was found more effective in higher concentration (50 ppm, 100 ppm). Therefore Vitavex is better to use for the management of Rhizoctonia solani in the field. 

Keywords
Poison food technique; In-vitro evaluation; Rhizoctonia solani; Chemicals; Maize

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. Manju Adhikari
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. Sundar Man Shrestha
. Shankar Prasad Gaire
. Basistha Acharya
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