Expression Analysis of Arabidopsis thaliana Genes ARGAH1ARGAH2 and the Response to Sodium Chloride Stress During Seed Germination  

Xiaoxu Zhang1 , Xinxin Zhang1 , Tetsuo Takano2 , Shenkui Liu1 , Yuanyuan Bu1
1. Key Laboratory of Saline-alkali Vegetation Ecology Restoration in Oil Field (SAVER), Ministry of Education, Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, Harbin 150040, China
2. Asian Natural Environmental Science Center (ANESC), The University of Tokyo, Tokyo 188-0002, Japan
Author    Correspondence author
Genomics and Applied Biology, 2014, Vol. 5, No. 2   doi: 10.5376/gab.2014.05.0002
Received: 18 Feb., 2014    Accepted: 21 Mar., 2014    Published: 29 Apr., 2014
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This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Zhang et al., 2014, Expression Analysis of Arabidopsis thaliana Genes ARGAH1ARGAH2 and the Response to Sodium Chloride Stress During Seed Germination, Genomics and Applied Biology, Vol.5, No.2, 1-6 (doi: 10.5376/gab.2014.05.0002)

Abstract

Arabidopsis thaliana possesses two arginase-encoding genes(ARGAH1ARGAH2), which catalyses the catabolism of arginine into L-ornithine and urea. In this research, we focus on the function of the two genes (ARGAH1ARGAH2) encoding Arabidopsis thaliana arginase. The cDNA of cloned ARGAH1 is 1347 bp, and 5' untranslated region (UTR) is 145 bp and the 3' UTR is 166 bp. The open reading frame of ARGAH1 is 1029 bp, encoding 342 amino acids. ARGAH2 is 1284 bp, and 5' untranslated region (UTR) is 34 bp and the 3' UTR is 215 bp. The open reading frame of ARGAH2 is 1035 bp, encoding 344 amino acids. We studied the different expression models of arginase genes under NaCl stress by qRT-PCR and other biotechnology. Interestly, we observed that the expression of ARGAH1 and ARGAH2 is both induced by NaCl stress. At the same time, the enzyme assay of argianse also showed an increase during seed germination. Therefore, we maintain that the argianse genes play a role against to NaCl stress especially during the germination.

Keywords
Arabidopsis thaliana; ARGAHs; Salt Stress; Seed Germination
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