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Cloning of an Ascorbate Peroxidase Gene from Puccinellia tenuiflora and its Expression Analysis | Guan 1,2 | Genomics and Applied Biology

Cloning of an Ascorbate Peroxidase Gene from Puccinellia tenuiflora and its Expression Analysis  

Qingjie Guan1,2 , Lin Li1 , Takano Tetsuo2 , Shenkui Liu1
1. Alkali Soil Natural Environmental Science Center (ASNESC), Northeast Forestry University, Harbin, 150040
2. Asian Natural Environment Science Center (ANESC), The University of Tokyo, Tokyo, 1880002, Japan
Author    Correspondence author
Genomics and Applied Biology, 2011, Vol. 2, No. 1   
Received: 10 Sep., 2010    Accepted: 11 Feb., 2011    Published: 25 Feb., 2011
© 2011 BioPublisher Publishing Platform

This article was first published in Genomics and Applied Biology (Regular Print Version) in Chinese, and here was authorized to redistribute in English under the terms of theCreative Commons Attribution License , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

The full-length gene of an ascorbate peroxidase (PutAPx) was isolated from Puccinellia tenuiflora Ohwi cDNA library. The gene is 1 125 bp in length and it has an ORF of 876 bp, which encoded a protein of 291 amino-acid with an estimated molecular weight 32 kD and an isoelectric point of 7.71. Blasting at NCBI, we found that PutAPx showed high similarity (89.7%, 94.3%, 94.2%, 50.7% and 79.6%) to 5 different gramineae species (Oryza sativa L., Hordeum vulgare L., Triticum aestivum, Lolium perenne L., and Zea mays). The result of phylogenetic tree showed that PutAPx has the closdest genetic distance with Hordeum vulgare L. and Triticum aestivum. Transgenic yeast (InVSC1), expressing PutAPx gene, under the inducement withβ-galactose showed higher stress resistance in oxidation stress than control. In this study, we successfully cloned an ascorbate peroxidase (PutAPx) and studied in primary levle, which laid the foundation for the future study in the mechanism of oxidative stress mechanism of the role of the foundation establish.
 

Keywords
Puccinellia tenuiflora Chinampoensis Ohwi; Ascorbate peroxidase; Gene cloning
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