Investigation of PVL and MecA Gene Locies in Methicillin-Resistant Staphylococcus aureus (MRSA) Strains Isolated from Nosocomial Samples of Hospital Patients and Hospital Staff  

Yaren H.1 , Saygin O.1 , Turaclar N.2 , Vural H.C.3 , Turhan A.B.3 , Tuncer E.I.4 , Arslan U.4
1 Department of Biology, Faculty of Science, Selcuk University, Konya, Turkey
2 Vocational School of Health Services, Selcuk University, Konya, Turkey
3 Faculty of Medicine, Medical Biology Department, Necmettin Erbakan University, Konya, Turkey
4 Faculty of Medicine, Microbiology Department, Selcuk University, Konya, Turkey
Author    Correspondence author
Genomics and Applied Biology, 2015, Vol. 6, No. 10   doi: 10.5376/gab.2015.06.0010
Received: 12 Nov., 2015    Accepted: 26 Dec., 2015    Published: 31 Dec., 2015
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Preferred citation for this article:

Yaren H., Saygin O., Turaclar N., Vural H.C., Turhan A.B., Tuncer E.I., and Arslan U., 2015, Investigation of PVL and MecA Gene Loci in Methicillin-Resistant Staphylococcus aureus (MRSA) Strains Isolated from Nosocomial Samples of Hospital Patients and Hospital Staff, Genomics and Applied Biology, 6(10): 1-6 (doi: 10.5376/gab.2015.06.0010)

Abstract

Objective: Methicillin-resistant (MRSA) is a bacterium responsible for some infections difficult to treat in humans. Antibiotic resistance is the ability of microorganisms to resist the effects of antibiotic. MRSA is a strain of Staphylococcus aureus that is resistant to a large group of antibiotics by its definition. We studied in the Archeometry and Biotechnology Laboratory of Science Faculty in Selcuk University. In this study, the samples were taken from cultured nosocomial infections of 233 hospital staff and 477 hospital patients (total 710) in the Medical School Hospital of Selcuk University. We collected 2 Methicillin-susceptible Staphylococcus aureus (MSSA) samples from hospital patients and 40 Methicillin-resistant Staphylococcus aureus (MRSA) samples from hospital patients and staff (36 hospital patients 7.5%, 4 hospital staff 1.7%) for this research. Methods: We looked into present of mecA-1, mecA-2, PVL-1, PVL-2, and PVL-3 genes in each samples by PCR methods. mecA-1 and mecA-2 gene loci or band patterns in 40 MRSA samples was amplified by PCR. Results: mecA-1 and mecA-2 were observed in 40 MRSA, but not observed in 2 MSSA. PVL-1 gene was observed in 22 MRSA, PVL-2 gene was observed in 25 MRSA, PVL-3 gene was observed in 33 MRSA. PVL gene region was not observed in 2 MSSA strains. Molecular findings were verified the microbiologic results in the research. The mutation was not determined in MRSA originated from hospital patients and staff. Conclusion: The result of this study showed that the prevalence of MRSA was high in the hospital. It is an important sign to meet the challenges of the fight against epidemics.

Keywords
MRSA; mecA; PVL; MSSA; Nosocomial samples
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