Research Article

Antioxidant Defense Response of Lentil (Lens culinaris L.) Mutants Impaired in Enzymes at Branching Point of Sulfate Assimilation  

Talukdar D.
Department of Botany, R.P.M. College, Hooghly, 712258, India
Author    Correspondence author
Genomics and Applied Biology, 2015, Vol. 6, No. 12   doi: 10.5376/gab.2015.06.0012
Received: 25 Sep., 2015    Accepted: 17 Jan., 2016    Published: 05 Apr., 2016
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This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Talukdar D., 2015, Antioxidant defense response of lentil (Lens culinaris L.) mutants impaired in enzymes at branching point of sulfate assimilation, India, Genomics and Applied Biology, 6(12): 1-11(doi: 10.5376/gab.2015.06.0012)

Abstract

Lentil (Lens culinaris Medik.) is an important protein rich pulse crop in the Indian subcontinent, West Asia, North Africa and parts of Europe, Oceania and North America. Sulfate assimilation constitutes a significant part in growth, yield and development of crop plants. However, our knowledge regarding sulfate assimilation in any grain legume is poor. Main aim of the present study was therefore to reveal the antioxidant defense response of lentil mutants which are impaired at either point of branches in sulfate assimilation. Two lentil mutants namely apkLc1 (deficient in adenosine 5´-phosphosulfate kinase activity) and aprLc1 (deficient in adenosine 5´-phosphosulfate reductase) were isolated in 0.15% ethyl methane sulfonate-treated M2 progeny of lentil cv. L 414. The apkLc1 mutant exhibited improved growth performances accompanied by stimulated antioxidant defense components like GSH and AsA-GSH cycle enzymes. Despite poor phenylalanine lyase (PAL) activity, the apkLc1 mutant did not suffer oxidative damage due to low level of H2O2 and lipid peroxidation. Contrastingly, aprLc1 mutant suffered oxidative damage and growth inhibition due to low AsA and GSH pool and reduced antioxidant defense. The result suggests partitioning of S between APR and APK in two different mutants in different ways and APR mutation was found more detrimental for plant growth performances, biomass accumulation and seed yields than APK mutation at branching point of S assimilation.

Keywords
APS reductase; APS kinase; Lentil mutants; Oxidative stress
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