Transcription of Rice Green Revolution Gene sd1 is Clarified by Comparative RNA Diagnosis Using the Isogenic Background  

Motonori Tomita1 , Shota Matsumoto2
1. Molecular Genetics Laboratory, Faculty of Agriculture, Tottori University, 101, Minami 4-chome, Koyama-cho, Tottori 680-8553, Japan
2. Molecular Genetics Laboratory, Faculty of Agriculture, Tottori University, Tottori 680-8553, Japan
Author    Correspondence author
Genomics and Applied Biology, 2011, Vol. 2, No. 5   doi: 10.5376/gab.2011.02.0005
Received: 14 Nov., 2011    Accepted: 29 Nov., 2011    Published: 20 Dec., 2011
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Tomita and Matsumoto, 2011, Transcription of Rice Green Revolution Gene sd1 is Clarified by Comparative RNA Diagnosis Using the Isogenic Background,  Genomics and Applied Biology , 2011, Vol. 2, No.5 (DOI: 10.5376/gab.2011.02.0005)

Abstract

The sd1 allele, on the long arm of chromosome 1, encoding a defective C20-oxidase in the gibberellin (GA) biosynthesis pathway(GA 20-oxidase, OsGA20ox2) confers the semidwarf phenotype thatcontributed to Green Revolution in rice. It has yet to be known whether sd1 is transcribed. The two alleles at the sd1/OsGA20ox2 locus of each line, the Japanese leading varieties Koshihikari and Hikarishinseiki having inherited over 99.8% of the Koshihikari genome, except for sd1, were successfully distinguished by RT-PCR amplification of the first exon followed by digestion with PmaCI. RNA extracted from the leaves and roots was employed as the template then the 779 bp fragment was clearly cleaved into the 613 bp and 166 bp fragments by PmaCI digestion in Hikarishinseiki, but not cleaved in Koshihkari. Therefore, the RNA diagnosis figured out clarified that sd1 gene derived from Jukkoku was transcribed in Hikarishinseiki. It would be a first evidence of the transcription of sd1. Moreover, the taste and quality of Hikarishinseiki were in line with that of Koshihikari across Japan during two years, and it might be practically valuable as a brand rice description in the rice-producing districts of 13 prefectures in Japan.

Keywords
Rice (Oryza sativa L); Semidwarf ; Green Revolution; sd1; Transcription; RNA diagnosis; Trait performance; Lodging resistance
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