Construction of the Near-Isogenic Lines of the MI CMS Restorer Gene in Rapeseed (Brassica napus) and Allelic Analysis  

Weihua Long , Maolong Hu , Jianqin Gao , Huiming Pu , Song Chen , Jiefu Zhang , Cunkou Qi
The Industry Crops Institute of Jiangsu Academy of Agriculture Science, Nanjing Branch of National Improvement Center of Rapeseed, Nanjing, 210014, P.R. China
Author    Correspondence author
Genomics and Applied Biology, 2011, Vol. 2, No. 6   doi: 10.5376/gab.2011.02.0006
Received: 20 Nov., 2011    Accepted: 09 Dec., 2011    Published: 16 Jan., 2012
© 2011 BioPublisher Publishing Platform
This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Long et al., 2011, Construction of the Near-Isogenic Lines of the MI CMS Restorer Gene in Rapeseed (Brassica napus) and Allelic Analysis, Genomics and Applied Biology, 2011, Vol.2, No.6 34-41 (doi: 10.3969/gab.2011.02.0006)

Abstract

In this research, we employed Ning R7, an elite restorer for MI CMS, as the restorer gene donor to develop restorer gene candidate near-isogenic lines (NILs) by using three elite germplasms, T78, T84 and ZS9, as recurrent parents based on the advanced backcross approach. The characteristics of agronomic traits were phenotyped at the maturity stage and genetic similarity was calculated based on the SSR data. The results showed most agronomic traits of the candidates had quite similar with that of recurrent parents besides the trait of plant height and the height of the initiate branch based on t-test.  Further, in order to figure out the similarity of the genetic background, we used 75 SSR markers to generate total 157 SSR loci in the 21 candidate NILs. The average genetic similarity coefficient of the candidate NIL background reached 0.86, 0.92 and 0.98 corresponding to their own recurrent parent, respectively. With the comprehensive assessment, three candidate NILs, wh13, wh59 and wh118, were chosen as potential restorer NILs, which might be used in our future breeding program.

Keywords
Rapeseed (Brassica napus L.); CMS; Restorer gene; Near-Isogenic Lines (NIL); Allelic analysis
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