The Acquisition and Expression Analysis of Polygalacturonase Gene MF6 Related to Fertility in Brassica napus L.  

Rongxian Huang , Zhikang Tang , Shixing Guo , Yingze Niu
Rapeseed Research Center, Sichuan Agricultural University, Chengdu, 611130, P.R.China
Author    Correspondence author
Genomics and Applied Biology, 2012, Vol. 3, No. 1   doi: 10.5376/gab.2012.03.0001
Received: 25 May, 2012    Accepted: 28 Jun., 2012    Published: 30 Jun., 2012
© 2012 BioPublisher Publishing Platform
This article was first published in Molecular Plant Breeding (2012, 10(3): 298-304) in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Huang et al., 2012, The Acquisition and Expression Analysis of Polygalacturonase Gene MF6 Related to Fertility in Brassica napus L., Genomics and Applied Biology, 2012, Vol.3No.1 1-7 (doi: 10.3969/gab.2012.03.0001)

Abstract

The purpose of this study is to analyze the differentially expressed genes in the buds of male sterile plants which are induced with a male sterilizing chemical "Hua-Sha-Ling WP1" in Brassica napus L., R121. Firstly, we isolated the differentially expressed gene fragments from the male sterile buds through the suppression subtractive hybridization (SSH) and the reverse northern dot blotting. Then, homology comparisons and functional analysis were conducted through online database NCBI. Finally, the differentially expressed gene was further analyzed through reverse transcription quantitative PCR. In the results, we obtained a gene fragment which was 100% homologous to the polygalacturonase gene (MF6) in Arabidopsis from the 6 positive clones. It is reported that the polygalacturonase gene (MF6) is closely related to the development and maturation of pollen. The amount of MF6 gene expression in flower buds with different lengths and different fertilities was analyzed quantitatively. The results showed that the expression of MF6 gene in the male fertile flower buds was higher than that in the male sterile flower buds. And the differences are about 18.53 times and 43.15 times when the bud lengths are 1.00~1.50 mm and 3.5~4.0 mm, respectively. Therefore, "Hua-Sha-Ling WP1" may inhibit the expression of the MF6 gene during the meiosis stages of pollen mother cell and cause the abortion of pollens. At the same time, the MF6 gene expression may also be associated with the elongation of filaments.

Keywords
Brassica napus L.; Chemical male sterilization; Differentially expressed genes; MF6; Pollen abortion
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