Olive Oil Generated by the Recombinant Escherichia coli with ACCase Gene from Jatropha curcas
Elsayed E. Hafez1
Salahedin G. Ali2
Elsayed K. Bakhiet2
1 City for Scientific Research and Technology Applications, Arid Lands and Development Research Institute, 21934, Alexandria, Egypt
2 Faculty of Science, Assuit University Alazher, Assuit, Egypt
Genomics and Applied Biology, 2013, Vol. 4, No. 2 doi: 10.5376/gab.2013.04.0002
Received: 09 Jan., 2013 Accepted: 11 Feb., 2013 Published: 08 Apr., 2013
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Preferred citation for this article:
Hafez et al., 2013, Olive oil generated by the recombinant Escherichia coli with ACCase gene from Jatropha curcas, Genomics and Applied Biology, Vol.4 No.2 8-14 (doi: 10.3969/gab.2013.04.0002)
Acetyl-CoA carboxylase (ACCase) has a very important regulatory role in controlling plant fatty acid biosynthesis, thereby affecting lipid biosynthesis. The ACCase gene was amplified from Jatropha curcas using PCR with two degenerate primers, and the 1250-bp amplicon was cloned and sequenced. Sequence analysis revealed that the sequence obtained was similar to Jatropha curcas, Ricinus communis, Camellia sinensis and Phaseolus vulgaris acetyl cocoa, with 75% similarity. The full length of the gene was sub-cloned into a prokaryotic expression vector and the induced recombinant Escherichia coli was grown on 0.2% (w/v) sodium oleate. The cell metabolite was analyzed using thin-layer chromatography and HPLC. This analysis revealed that the cell metabolite consisted of a mixture of esters, mainly consisting of oleic acid (0.7 g/L) plus minor amounts of palmitic acid, linoleic acid and stearic acid. Fed-batch cultivation of E. coli was conducted at the specific growth rates of 0.15 and 0.1 h-1 for constant and exponential strategies, respectively. A high cell density of 20 g/L with an overall biomass yield of 3 g/L was achieved.
Acetyl Co-A carboxylase gene; Jatropha curcas; Biodiesel; Oleic acid; Palmitic acid; Linoleic acid; Stearic acid