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Genetic Diversity of Cyprinus carpio var. communis , Cyprinus carpio var. specularis and Carassius carassius by DNA Based Markers | Wali 1 | International Journal of Aquaculture

Genetic Diversity of Cyprinus carpio var. communis, Cyprinus carpio var. specularis and Carassius carassius by DNA Based Markers  

Asifa Wali1 , Mudasir Syed2 , Bilal Ahmad Bhat1 , Masood-ul Hassan Balkhi1 , Farooz Ahmad Bhat1 , Mohd Maqbool Darzi3
1 Faculty of fisheries Sher-e-Kashmir University of Agricultural Sciences and Technology, Kashmir, Shuhama Srinagar -19006, India
2 Division of Biotechnology, Faculty of Veterinary Sciences and Animal Husbandry, Shuhama. Sher-e- Kashmir University of Agricultural Sciences and Technology, Kashmir, Shuhama Srinagar -19006, India
3 Division of Pathology, Faculty of Veterinary Sciences and Animal Husbandry, Shuhama. Sher-e-Kashmir University of Agricultural Sciences and Technology, Kashmir, Shuhama Srinagar -19006, India
Author    Correspondence author
International Journal of Aquaculture, 2013, Vol. 3, No. 24   doi: 10.5376/ija.2013.03.0024
Received: 29 Jul., 2013    Accepted: 22 Aug., 2013    Published: 02 Oct., 2013
© 2013 BioPublisher Publishing Platform
This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Wali et al., 2013, Genetic Diversity of Cyprinus carpio var. communis, Cyprinus carpio var. specularis and Carassius carassius by DNA Based Markers, International Journal of Aquaculture, Vol.3, No.24 138-146 (doi: 10.5376/ija.2013. 03.0024)

Abstract

To study genetic relationship of Cyprinus carpio var. communis, Cyprinus carpio var. specularis and Carassius carassius collected from different locations of Kashmir, India, two molecular approaches viz. PCR-RFLP of Cyt b gene and random amplified polymorphic DNA (RAPD) were used. Twenty five samples for each of the species collected from different locations of Kashmir, India were used for this study. Of the 12 random primers used 8 generated the polymorphism, while as four out of 7 restriction enzymes used gave polymorphic restriction patterns. The average proportion of polymorphic markers across restriction endonucleases was 74.4%, whereas random primers showed 82.23% polymorphism. The study showed RAPD marker efficiently helped in inter- and intra-species variation of the species. In contrast PCR- RFLP showed bias in detecting intraspecific variations. However, it was found an efficient tool for inter-specific variation between the species under study. The unweighted pair group method with averages (UPGMA) was used to construct two separate dendrogram for RAPD and PCR-RFLP which resulted in two clusters, one containing C. c. communis and C. c. specularis and the other included the C. carassius species. The Phylogenetic analysis demonstrated that scale carp (C. c. communis) is closest to mirror carp (C. c. specularis) than crucian carp (Crassius carassius). Mean values of haplotype and nucleotide diversity across the species were 0.0200 and 1.0000±0.0113, respectively. Adding more populations with increased sample size of each species will be imperative to draw more definitive conclusions about species characterization within this region.

 

Keywords
Cyprinidae; Genetic diversity; RAPD; PCR-RFLP; Cyt b
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