Micropropagation and in Vitro Flowering of an Ornamental Aquarium Plant Lindernia antipoda L. (Alston)
Sree Lakshmi S.
Department of Marine Biology, Microbiology & Biochemistry, School of Marine Science, Cochin University of Science and Technology, Kerala, India
International Journal of Aquaculture, 2016, Vol. 6, No. 8 doi: 10.5376/ija.2016.06.0008
Received: 29 Feb., 2016 Accepted: 12 Apr., 2016 Published: 23 Sep., 2016
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Preferred citation for this article:
Jabir T., Sheeja George., Anjana Raj., Sree Lakshmi S., Aneykutty Joseph., 2016, Micropropagation and in vitro flowering of an ornamental aquarium plant Lindernia antipoda (L.) Alston, 6(8): 1-10 (doi: 10.5376/ija.2016.06.0008)
Lindernia antipoda L. (Alston) is one of the valuable aquatic ornamental plants which have a bright potential in the aquarium trade. In the present study a protocol for rapid shoot multiplication and in vitro flowering from axillary bud was developed. Half MS media with 1 mg/L benzylaminopurine (BAP) was the found to be the best medium for culture initiation and establishment. The effective medium for shoot multiplication was MS+1 mg/L BAP+0.2 mg/L NAA. In this media, 85% of the cultures exhibited multiple shoot proliferation. The highest shoot proliferation and shoot length were also acquired in this media. Plant showed highest in vitro flowering in MS medium supplemented 1 mg/L BAP and 0.2 mg/L α-Naphthalene acetic acid (NAA). All concentrations of NAA induced rooting. Hardening of in vitro cultured plantlets were attained through polythene propagator system and 90% of the plants survived, and it was successfully transformed in to an aquarium.
Lindernia antipoda; Aquarium plant; Multiple shoot proliferation; In vitro flowering