Screening of Cellulose - Degrading Bacteria Associated with Gastrointestinal Tract of Hybrid Abalone as Probiotic Candidates
Muhamad Amin1, 2
1 Institute for Marine and Antarctic Studies (IMAS), University of Tasmania, Launceston, Tasmania 7250, Australia
2 Fisheries Faculty, University of 45 Mataram, West-Nusa Tenggara, Indonesia
International Journal of Aquaculture, 2016, Vol. 6, No. 10 doi: 10.5376/ija.2016.06.0010
Received: 10 Oct., 2016 Accepted: 08 Nov., 2016 Published: 20 Nov., 2016
© 2016 BioPublisher Publishing Platform
This is an open access article published under the terms of the Creative Commons Attribution License
, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:
Amin M., 2016, Isolation and characterization of cellulose-degrading bacteria from gastrointestinal tract of hybrid abalone as probiotic candidates, International Journal of Aquaculture, 6(10): 1-9 (doi: 10.5376/ija.2016.06.0010)
This study aimed at isolation and characterization of cellulose - degrading bacteria from gastrointestinal tracts (GIT) of hybrid abalone (Haliotis laevigata x H. rubra) as aquaculture probiotic candidates. The result showed that 7 endosymbiont bacteria had a capacity to degrade cellulose. Of these, 2 isolates exhibiting the highest cellulolytic activity were isolate C - aw2 and C -1. Based on their 16S rDNA sequences, these bacterial isolates displayed high similarity to Stenotrophomonas sp strain LY -2, and Bacillus sp strain WR -2, for isolate C – aw2 and C -1 respectively. Further in vitro characterizations indicated that these bacteria had a high viability in seawater and simulated GIT environments. In addition, both bacteria were non - toxic to juvenile abalone determined through an in vivo study. These results suggest that Stenotrophomonas sp and Bacillus sp could be potential probiotic candidates, especially to increase digestibility of plant-based aquaculture diets. However, the effect of these bacterial supplementation on the food digestibility of live aquatic species need to be further investigated.
Bacillus sp; Cellulolytic activity; Probiotic candidates; Stenotrophomonas sp