Research Article

Efficiency of Indigenous Pseudomonas aeruginosa as Biocontrol Agent against Vibrio Infection in Shrimp (Penaeus monodon) Culture  

C.N. Ariole , N.G. Anyanwu
Department of Microbiology, University of Port Harcourt, P.M.B. 5323, Port Harcourt, Rivers State, Nigeria
Author    Correspondence author
International Journal of Aquaculture, 2017, Vol. 7, No. 3   doi: 10.5376/ija.2017.07.0003
Received: 29 Dec., 2016    Accepted: 23 Jan., 2017    Published: 06 Feb., 2017
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This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Ariole C.N., and Anyanwu N.G., 2017, Efficiency of indigenous Pseudomonas aeruginosa as biocontrol agent against Vibrio infection in shrimp (Penaeus monodon) culture, International Journal of Aquaculture, 7(3): 15-22 (doi: 10.5376/ija.2017.07.0003)

Abstract

The efficacy of Pseudomonas aeruginosa as a biocontrol agent against Vibrio mimicus infection in shrimp (Penaeus monodon) culture was evaluated. The antagonistic Pseudomonas sp. Ps2 and Vibrio sp. V1 were previously isolated from healthy and moribund shrimp intestines respectively. The two strains were identified using molecular method. Four-day acute pathogenicity tests were carried out on shrimp (Penaeus monodon) postlarvae using different concentrations of the strains. Histopathological examination of the hepatopancreas of the postlarvae was carried out after each pathogenicity test. Probiotic trial of the antagonistic Pseudomonas aeruginosa on culture of shrimp (Penaeus monodon) challenged with pathogenic Vibrio mimicus by immersion was carried out for 12 days. Water quality parameters (pH, temperature, ammonia, nitrite, nitrate, salinity and dissolved oxygen) were determined during shrimp culture. Molecular analysis conducted on Pseudomonas sp. Ps2 and Vibrio sp. V1 revealed that they are closely related to Pseudomonas aeruginosa strain XyC5 and Vibrio mimicus strain XQ respectively. The use of Pseudomonas aeruginosa had no pathogenicity effect on shrimp since no shrimp mortality was recorded. The LC50 of Vibrio mimicus was calculated to be 3.2 x 104 cfu/ml. Vibrio mimicus addition resulted in 54% mortality and unhealthy hepatopancreas. No addition of bacteria resulted in 22% mortality and healthy hepatopancreas while Pseudomonas aeruginosa plus Vibrio mimicus addition resulted in zero mortality and healthy hepatopancreas. The water quality parameters were within the optimum ranges throughout the culture period. The results suggest that the indigenous antagonistic Pseudomonas aeruginosa strain XyC5 could be beneficial as biological control agent for management of vibriosis in shrimp aquaculture.

Keywords
Probiotic; Vibrio infection; Shrimp; Hepatopancreas; Water quality
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