Microsatellite Marker Development and Characterization in the Spotted Babylon, Babylonia areolata (Link, 1807): Detection of Duplicated Loci at High Frequency  

Nan Zhang* , Ying Qiu* , Xuzhen Huang , Xuefen Chen , Aimin Wang , Yan Wang
Key Laboratory of Tropical Biological Resources of Ministry of Education, Hainan Provincial Key Laboratory of Tropical Hydrobiology and Biotechnology, Marine Biology Experiment Teaching Demonstration Center, Ocean College, Hainan University, 58 Renmin Rd, Haikou, 570228, P.R. China
* The authors who contribute equally
Author    Correspondence author
International Journal of Aquaculture, 2012, Vol. 2, No. 2   doi: 10.5376/ija.2012.02.0002
Received: 15 Mar., 2012    Accepted: 06 Apr., 2012    Published: 14 May, 2012
© 2012 BioPublisher Publishing Platform
This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Zhang et al., 2012, Microsatellite Marker Development and Characterization in the Spotted Babylon, Babylonia areolata (Link, 1807): Detection of Duplicated Loci at High Frequency, International Journal of Aquaculture, Vol.2, No.2 5-10 (doi: 10.5376/ija.2012. 02.0002)

Abstract

Four polymorphic microsatellite markers were developed for the spotted babylon, Babylonia areolata, from a microsatellite enriched library. Those markers, characterized in 32 individuals from one wild population, were polymorphic with allele numbers ranging from 5 to 15 per locus, expected and observed heterozygosity ranging from 0.60 to 0.92 and from 0.36 to 0.88, respectively. One locus showed significant (p<0.05 after Bonferroni correction) deviation from Hardy–Weinberg equilibrium, probably due to the presence of null alleles. These microsatellite markers should be useful for population genetics studies in this species. In addition, nine primer pairs amplified duplicated microsatellites, with four multiple loci clearly linked in tandem, which provides a good opportunity to study the evolution of repetitive DNA sequences in the genome.

Keywords
Babylonia areolata; Microsatellites; Duplicated loci; Population genetics; Fishery; Gastropod; Spotted babylon
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