Multiplication rate of selected potato cultivars in vitro through single node culture  

Jane Muthoni , Christine Muchira , J. N. Kabira
Kenya Agricultural Research Institute (KARI). National Potato Research Centre, Tigoni, Kenya
Author    Correspondence author
International Journal of Horticulture, 2014, Vol. 4, No. 15   doi: 10.5376/ijh.2014.04.0015
Received: 01 Aug., 2014    Accepted: 17 Sep., 2014    Published: 30 Oct., 2014
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This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Muthoni et al., 2014, Multiplication rate of selected potato cultivars in vitro through single node culture, International Journal of Horticulture, 2014, Vol.4, No.15 1-4 (doi: 10.5376/ijh.2014.04.0015)

Abstract

A study was carried out in the tissue culture laboratory at the National Potato Research Centre (NPRC), Tigoni, Kenya to determine the multiplication rate of selected potato cultivars when micropropagated through single node culture. Seven potato cultivars were subcultured on solid media for three consecutive cycles (each cycle took three weeks). This was repeated twice. Data collected was the number of single-node cuttings that were generated from each node after subculturing. Generally, there were significant differences (P≤0.05) on the number of single node cuttings generated among the potato cultivars, among the subculture cycles and in the interaction between genotypes and subculture cycle.

Keywords
Micropropagation; Potatoes; Single-node; Subculture
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