Toxicological and Histopathological Effects of Cheese Wood, Alstonia boonei De Wild Stem Bark Powder used as Cowpea Protectant against Cowpea Bruchid, Callosobruchus maculatus (Fab.) [Coleoptera: Chrysomelidae] on Albino Rats.  

Kayode David Ileke1 , Olusola Olasumbo  Odeyemi2 , Michael  Olufemi  Ashamo2
1. Department of Environmental Biology and Fisheries, Faculty of Science, Adekunle Ajasin University, PMB 001, Akungba-Akoko, Ondo State, Nigeria
2. Department of Biology, School of Science, Federal University of Technology, PMB 704, Akure, Ondo State, Nigeria
Author    Correspondence author
International Journal of Molecular Medical Science, 2014, Vol. 4, No. 2   doi: 10.5376/ijmms.2014.04.0002
Received: 20 Mar., 2014    Accepted: 10 May, 2014    Published: 17 Jun., 2014
© 2014 BioPublisher Publishing Platform
This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract
Toxicological and histopathological effects of Alstonia boonei stem bark powder on albino rats liver and kidney functions were investigated using standard methods. After two weeks of acclimatization, the rats were randomly divided into four (I - IV) groups of six animals each. Group I was fed with Basal diet, group II - IV were for 30 days with basal diet containing 1%, 4% and 10% A. boonei stem bark powder, respectively. Blood was rapidly collected by direct heart puncture and the plasma aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), total protein, urea and creatinine contents were determined using commercially-available kits. The levels of these biochemical functions were also determined in the liver and kidney. The A. boonei stem bark powder at 10% concentration caused oxidative liver and kidney damages. Histopathological results of the liver shows that the group fed with 4% A. boonei stem bark powder had normal hepatocytes. The group fed with 10% A. boonei stem bark powder had necrosis. The results of the present study showed that the supplementation of 1% and 4% A. boonei stem bark powder to the diets of rats for 30 days did not change the biochemical parameters of liver and kidney as well as histopathological investigation which illustrated normal architecture of liver and kidney.
Keywords
Alstonia boonei; Albino rats; Callosobruchus maculatus; Cowpea seed; Toxicology; Histopathology
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