Research Article

The Isolation of Metabolite Compounds from Seaweed (Halimeda gracillis) in the Waters of Teluk Lampung as a Source of Antibacterial Compounds  

Muhammad Hendri1 , J.S. Darmanto2 , Budi Prayitno2 , Ocky Karna Radjasa3 , Elvita 4
1 Department of Marine Science, FMIPA Sriwijaya University, Palembang 30662, South Sumatera, Indonesia
2 Jurusan Teknologi Hasil Perikanan FPIK Universitas Diponegoro, Indonesia
3 Department of Marine Science, Diponegoro University, Semarang 50275, Indonesia
4 Department of Chemist, FMIPA Sriiwijaya University, Indonesia
Author    Correspondence author
International Journal of Marine Science, 2017, Vol. 7, No. 31   doi: 10.5376/ijms.2017.07.0031
Received: 14 Jul., 2017    Accepted: 07 Aug., 2017    Published: 11 Aug., 2017
© 2017 BioPublisher Publishing Platform
This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Hendri M, Darmanto J.S, Prayitno B, Radjasa O.K, and Elvita, 2017, The isolation of metabolite compounds from seaweed (Halimeda gracillis) in the waters of Teluk Lampung as a source of antibacterial compounds, International Journal of Marine Science, 7(31): 297-307 (doi: 10.5376/ijms.2017.07.0031)

Abstract

The isolation of secondary metabolites of seaweed Halimeda gracilis was conducted. Isolation begins with 2 kg macerated dried powder Halimeda gracillis in 8 L of n-hexane solvent followed by ethyl acetate and methanol for 3 days. The solvent was filtered and followed by evaporation. Separation and purification of the isolated compounds were done by using chromatography technique. Antibacterial activity was tested by the disc diffusion method with the bacteria E. coli, S. disentriae, and B. subtilis. Isolated compounds are in the form of white crystals. Based on the spectroscopy analysis data of UV, IR, 1H-NMR, 13C-NMR, HMQC, HMBC, and COSY, the pure compounds isolated are β-sitosterol from n-hexane extract and oleic acid from ethyl acetate extract. Isolated compounds and n-hexane extracts showed non antibacterial active, while the ethyl acetate extract showed weak antibacterial active on three test bacteria at a concentration of 200,000 ppm with clear zones of 9.47; 11.09; and 8.84 mm.

Keywords
Halimeda gracillis; Antibacterial
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International Journal of Marine Science
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