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Research Report

Genetic Diversity of GIFT Tilapia Populations by AFLP Analysis  

Tao Yan , Yusong Guo , Zhongduo Wang , Li Liu , Chuwu Liu
Key Laboratory of Aquaculture in South China Sea for Aquatic Economic Animal of Guangdong Higher Education Institutes, Fisheries College, Guangdong Ocean University, Zhanjiang, 524025
Author    Correspondence author
International Journal of Marine Science, 2014, Vol. 4, No. 60   doi: 10.5376/ijms.2014.04.0060
Received: 25 Jul., 2014    Accepted: 05 Sep., 2014    Published: 24 Oct., 2014
© 2014 BioPublisher Publishing Platform
This article was first published in Genomics and Applied Biology (2014, 33: 3454-3462) in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Yan T., Guo Y.S., Wang Z.D., Liu L., and Liu C.W., 2014, Genetic diversity of GIFT tilapia populations by AFLP analysis, International Journal of Marine Science, 4(60): 1-6 (doi: 10.5376/ijms.2014.04.0060)

Abstract

The experiment was conducted to assess the genetic diversity of genetically improved farmed tilapia (GIFT) in order to evaluate and preserve this strain as a germplasm resource. The experiment study focused on the genetic diversity of the newly bred F16 population of GIFT tilapia obtained from Guanghui Farm Limited Company, Huazhou (Guangdong, China) was analyzed using AFLP. 187 bands were detected with 5 pairs of AFLP primers (E-AGG/M-CTT, E-AGG/M-CTG, E-AAC/M-CAG, E-ACA/M-CTG, E-ACA/M-CAG) based on 27 individuals, and among them 163 (87.17%) were polymorphic with an average of 32.6 bands for per AFLP primer. Average Nei’s gene diversity was 0.288,6, average Shannon’s information index was 0.433,9. These results showed that the experiment group deposit had rich genetic diversity and maintained a high genetic heterozygosity, had further breeding and development value.

Keywords
GIFT tilapia; AFLP; Genetic diversity; Proportion of polymorphic loci
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International Journal of Marine Science
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