Research Report

Transcriptome Sequence of Litopenaeus vannamei Based on High-throughput Sequencing  

Xiaohan Chen , Digang Zeng , Xiuli Chen , Daxiang Xie , Yongzhen Zhao , Chunling Yang , Ning Ma , Yongmei Li
Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Guangxi Institute of Fisheries, Nanning, 530021, China
Author    Correspondence author
International Journal of Marine Science, 2014, Vol. 4, No. 62   doi: 10.5376/ijms.2014.04.0062
Received: 25 May, 2014    Accepted: 07 Jul., 2014    Published: 24 Jul., 2014
© 2014 BioPublisher Publishing Platform
This article was first published in Genomics and Applied Biology (2013, 32(3): 303-318) in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Chen X.H., Zeng D.G., Chen X.L., Xie D.X., Zhao Y.Z., Yang C.L., Ma N., and Li Y.M., 2018, Transcriptome sequence of Litopenaeus vannamei based on high-throughput sequencing, International Journal of Marine Science, 4(62): 1-7 (doi: 10.5376/ijms.2014.04.0062)

Abstract

Litopenaeus vannamei shrimp is the most extensively farmed crustacean species in the world. However, the genome and transcriptome data of Litopenaeus vannamei is still relatively lacking. To obtain transcriptome information of Litopenaeus vannamei, the transcriptome of Litopenaeus vannamei hepatopancreas was sequenced by 454 high-throughput sequencing technology. A total of 500,177 ESTs were produced, with an average length of 363 bp. De novo assembly of ESTs data generated 20,225 unigenes between 50 and 8,980 bp in length, with an average length of 507 bp. Similarity searches against the NCBI-Nr database revealed that 13,676 (68%) of these unigenes have significant matches (E-value<10-5). In addition, the unigenes were similarity search against the GO, COG and KEGG databases, and were annotated with gene functional descriptions, gene ontology terms, or pathways. By high-throughput sequencing, we obtained abundant transcriptome information that could contribute to novel gene identification and genome research in Litopenaeus vannamei.

Keywords
High-throughput sequencing; Litopenaeus vannamei; Transcriptome
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