Effect of Temperature and Nutrient Limitation on the Growth and Lipid Content of Three Selected Microalgae (Dunaliella tertiolecta, Nannochloropsis sp. and Scenedesmus sp.) for Biodiesel Production  

Nita Rukminasari
Department of Fisheries, Marine Science and Fisheries Faculty, Hasanuddin University, Jl. Perintis Kemerdekaan Km. 10, Makassar - 90245, South Sulawesi, Indonesia
Author    Correspondence author
International Journal of Marine Science, 2013, Vol. 3, No. 17   doi: 10.5376/ijms.2013.03.0017
Received: 18 Mar., 2013    Accepted: 07 Apr., 2013    Published: 06 Apr., 2013
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This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Rukminasari, 2013, Effect of Temperature and Nutrient Limitation on the Growth and Lipid Content of Three Selected Microalgae (Dunaliella tertiolecta, Nannochloropsis sp. and Scenedesmus sp.) for Biodiesel Production, International Journal of Marine Science, Vol.3, No.17 135-144 (doi: 10.5376/ijms.2013.03.0017)

Abstract

Microalgae is one of potential source for biodiesel due to high efficiency of solar energy conversion to chemical energy. Several microalgae also have high lipid content per dry weight of biomass. The aims of the present work to study the effects of temperature and nutrient depletion on the growth and lipid content of three selected microalgae (Dunaliella tertiolecta, Nannochloropsis sp.  and Scenedesmus sp.) in view of their possible utilization as raw materials for biodiesel production. In addition, various lipid analysis methods were applied, such as gravimetric, Nile Red staining and FTIR spectroscopy. Algal growth and lipid content was strongly influenced by the variation of tested parameters; indeed, an increase or decrease temperature from ambient temparature and nutrient depletion practically increase in lipid content. Nile Red staining and FTIR spectroscopy are effective tool to analyse rapidly of lipid content from selected microalgae.

Keywords
Biodiesel; Dunaliella tertiolecta; Nannochloropsis sp.; Scenedesmus sp.; Lipid; Nile Red staining; FTIR spectroscopy
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