Oocyte Retrieval Methods, Grade and Percentage of Oocytes in Goats  

L. Ram Singh1 , K. Sadasiva Rao2 , K. Murali Mohan3
1. Assistant Professor, Department of Animal Reproduction, Gyanecology and Obstetrics
2. Professor & Univ. Head, Dept. of VGO, CVSc, Rajendranagar, Hyderabad- 500 030 (A.P.) INDIA
3. Assistant Professor, Dept. of VGO, CVSc, Rajendranagar, Hyderabad- 500 030 (A.P.) INDIA
Author    Correspondence author
International Journal of Molecular Veterinary Research, 2013, Vol. 3, No. 2   doi: 10.5376/ijmvr.2013.03.0002
Received: 15 Feb., 2013    Accepted: 04 Mar., 2013    Published: 21 Mar., 2013
© 2013 BioPublisher Publishing Platform
This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Singh et al., 2013, Oocyte Retrieval Methods, Grade and Percentage of Oocytes in Goats, International Journal of Molecular Veterinary Research, Vol.3, No.2 4-6 (doi: 10.5376/ ijmvr.2013.03.0002)

Abstract

The present study was undertaken to assess the relative efficiency of three different collection techniques of percentage and grades of oocytes in goats of Hyderabad. The mean oocyte recovery from aspiration technique, 2.15 ± 0.31 and 2.9 ± 0.46 in dissection technique and 6.55 ± 0.57 and 4.51 ± 0.46 in slicing technique. The mean oocyte recovery rate was 1.5 ± 0.22 and 1.0 ± 0.33 under aspiration technique, 2.58 ± 0.47 and 2.43 ± 0.36 under dissection technique and 5.26 ± 0.46 and 6.3 ± 0.71. The mean numbers of oocytes of different grades recovered were 0.34 ± 0.03, 0.62 ± 0.07 and 1.37 ± 0.09 in aspiration, dissection and slicing techniques, respectively. Among the different grades of oocytes, significantly higher per cent of A (27.78) and B (38.89) grade oocytes were retrieved by aspiration and dissection technique when compared to slicing technique. With respect to C and D grade oocytes, significantly higher numbers of oocytes were retrieved by slicing technique than the aspiration and dissection techniques. Out of 437 oocytes retrieved in the present study, significantly higher per cent (50.57) yield was by slicing technique, followed by dissection technique (37.07) and aspiration (12.53).

Keywords
Oocyte grading; Percentage; Aspiration; Dissection; Slicing; Goat
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