Prolactin gene polymorphism and its associations with milk production traits in Frieswal cow
Nusrat Nabi Khan
A. K. Das
Gulzar Ahmad Raher
Division of Animal Genetics and Breeding, Faculty of Vety. Sci. & A. H. R. S. Pura, Jammu
International Journal of Molecular Zoology, 2013, Vol. 3, No. 3 doi: 10.5376/ijmz.2013.03.0003
Received: 06 Jan., 2013 Accepted: 15 Jan., 2013 Published: 30 Jan., 2013
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Preferred citation for this article:
Bukhari et al., 2013, Prolactin Gene Polymorphism and Its Associations with Milk Production Traits in Frieswal Cow, Intl. J. of Molecular Zoology, Vol.3, No.3, 10-13
In the present study, polymorphism of PRL gene at exon-3 and its association with milk production traits in Frieswal cattle was investigated. Prolactin (PRL) gene exert multiple effects on the mammary gland include mammogenesis, lactogenesis and galactopoiesis. In order to evaluate the PRL gene polymorphism, we used the restriction fragment length polymorphism (RFLP) method. Blood samples were collected from randomly chosen 54 Frieswal lactating cows. Genomic DNA was extracted from venous blood by the method of John et al (1991) with slight modification and amplified by polymerase chain reaction technique. The PRL gene of the Frieswal cattle was amplified to produce a 156 bp fragment. The PCR products were electrophoresed on 2% agarose gel and stained by ethidium bromide. Then they were digested of amplicons with Rsa I, which revealed two alleles A and B. Data were analyzed using Pop Gene Popgene 32 software package and association was analyzed by simple analysis of variance model. In this population, AA, AB, and BB genotypes were identified with 0.315, 0.629 and 0.056 frequencies, respectively. Allele frequencies of A and B were 0.630 and 0.370, respectively. The significant (P<0.05) chi-square value in Frieswal cattle breeds showed that the studied population was not in Hardy-Weinberg equilibrium. It is concluded from the results of present study that the animals of BB genotype for higher lactation length and AB genotype for lactation yield and animals of AA genotype for minimum service period may be selected for future breeding.
Frieswal; Milk production traits; PCR-RFLP; Polymorphism; Prolactin gene