In vitro Studies on Cellular Mediated Immune Response in Haemocytes of Crab— Episesarma tetragonum  

Jeyachandran Sivakamavalli , Perumal Rajakumaran , Baskaralingam Vaseeharan
Crustacean Molecular Biology and Genomics Lab, Department of Animal Health and Management, Alagappa University, Karaikudi 630 004, Tamil Nadu, India
Author    Correspondence author
International Journal of Molecular Zoology, 2013, Vol. 3, No. 7   doi: 10.5376/ijmz.2013.03.0007
Received: 26 Aug., 2013    Accepted: 27 Sep., 2013    Published: 17 Oct., 2013
© 2013 BioPublisher Publishing Platform
This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Sivakamavalli et al., 2013, In vitro Studies on Cellular Mediated Immune Response in Haemocytes of Crab—Episesarma tetragonum, Int'l J. Mol. Zoo., Vol.3, No.7, 24-31 (doi:10.5376/ijmz. 2013. 03.0007)

Abstract

Studying the biological interaction with the invertebrate immune system is attractive for the advancement of a basic knowledge on invertebrate and vertebrate general immunity, because it offers various possible alternatives for disease management. The invertebrate immune system involves encapsulation, nodule formation and phagocytosis process by haemocytes (cellular mediated). In the present study, we investigated the morphology of the haemocytes of Episesarma tetragonum, their role in cellular mediated immune responses such as encapsulation, phagocytosis and viability assays. Encapsulation response of haemocytes is tested with the CM cellulose, Sepharose CL-6B and DEAE Sepharose 6CLB beads. DEAE Sepharose 6CLB beads showed (80%) good cellular response. Phagocytic activity of haemocytes showed pathogen engulfing activity against the microbial patterns (N-acteyl muramic acid (NAM), N-acetyl glutamic acid (NAG), lipopolysaccharide, β-1,3-glucans, chitins, laminarins, zymosan and peptidoglycans) present on the surface of the pathogens. Phagocytic activities of the haemocytes were decreased when increasing the incubation time. Viability of haemocytes was decreased when the time intervals of absorption of tryphan blue were increased. The present investigation enlightens the role of hemocytes in the cellular mediated immune responses of crustacean crab including all invertebrates.

Keywords
Episesarma tetragonum; Cell mediated responses; Viability; Phagocytosis
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