Research Report

Susceptibility Status of Culex quinquefasciatus to Alphacypermethrin, Bifenthrin and Bendiocarb in Gombe Metropolis  

E. Abba , K. P.  Yoriyo , J.  Philimon , J. Ayuba , B.S.  Abdulmalik , H. Saidu
Department of Biological Sciences, Faculty of Science, Gombe State University, PMB 127, Gombe, Nigeria
Author    Correspondence author
Journal of Mosquito Research, 2016, Vol. 6, No. 18   doi: 10.5376/jmr.2016.06.0018
Received: 10 Apr., 2016    Accepted: 22 Jun., 2016    Published: 07 Aug., 2016
© 2016 BioPublisher Publishing Platform
This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Abba E., Yoriyo K.P., Philimon J., Ayuba J., Abdulmalik B.S., and Saidu H., 2016, Susceptibility Status of Culex quinquefasciatus to Alphacypermethrin, Bifenthrin and Bendiocarb in Gombe Metropolis, Journal of Mosquito Research, 6(18): 1-6 (doi: 10.5376/jmr.2016.06.0018

Abstract

Six genera of culicines mosquitoes are known as vectors of several diseases such as yellow fever, dengue and filariasis. Insecticides are commonly used in controlling these vectors either through impregnated nets or by spraying. Susceptibility status of culicine to three different insecticides in Gombe metropolis was carried out between the months of march and may 2011 using the WHO protocol. A total of 1500 Adult female culicines were used for the tests. Mosquitoes from Gombe State University showed 96%, 63%, and 66% susceptibility to Alphacypermethrin (0.75%), Bifenthrin (0.15%) and Bendiocarb (0.1%) respectively. The mosquito samples from Dawaki quarters indicated 92%, 62% and 69% susceptibility to Alphacypermethrin, Bifenthrin, and Bendiocarb respectively; whereas culicines from New market Area showed susceptibility of 90% to Alphacypermethrin, 44% and 69% to Bifenthrin and Bendiocarb respectively. No full susceptibility to any of the chemicals was observed; however, percentage mortality to Alphacypermethrin in all the sites suggests the possibility of resistance that needs to be confirmed but results for Bifenthrin and Bendiocarb indicates resistance of the mosquitoes in all the study sites. In view of this, it is important to implement insecticide resistance monitoring strategy in Gombe State.

Keywords
Susceptibility; Alphacypermethrin; Bifenthrin; Bendiocarb; Gombe

1 Introduction

Mosquitoes belong to the order Diptera, family Culicidae, with many sub-families and many genera. The most common genera are Culex, Aedes and Anopheles. The sub-families Culicidae (culicines) contain 34 genera of mosquitoes of which medically important ones are Culex, Psorophora, Mansonia, Haemagogus and Sabethes. The genera Culex, Aedes and Mansonia are found in both tropical and temperate regions, whereas Psoropora spp are found only in North and South America (Jordan, 2007).

 

Mosquitoes can be controlled by adopting the following general methods: personal protection by covering all exposed parts of the body; elimination of breeding sites; taking preventive medicine or by use of chemicals to destroy the larvae or adults of the mosquitoes (Kotpal, 2010).

 

Insecticides are commonly used in controlling the mosquito vectors. The insecticides can be applied to nets- Insecticide Treated Nets (ITNs). Most of the nets currently in use are Long Lasting Insecticidal Nets (LLINs) or by spraying the insecticides on the walls or roof of houses and shelters of domestic animals in a given area in order to kill the adult mosquitoes that land and rest on such surfaces. The latter method is called Indoor Residual Spraying (IRS) (Kelly-hope et al., 2008).

 

There are four classes of insecticides used in IRS: Organochlorines- these are insecticides that contain carbon, hydrogen and chlorine. They are now primarily of historic interest, since few survive in today’s arsenal. E.g., DDT, taxophene, strobane. Organophosphates- contain phosphorous and work by inhibiting certain enzymes- cholinesterase (chE). E.g., temephos, bidrin, disulfoton, malathion. Carbamates- are derivatives of carbamic acid, their mode of action is also by inhibiting chE. E.g., Bendiocarb, adoxycarb, methiocarb, etc. Pyrethroids- they apparently work by keeping open the sodium channels in neuronal membranes. E.g., tetrametrin, bifenthrin, alphacypermeethrin (George et al., 2004).

 

Dichlorodiphenyltrichloroethane (DDT), malathion, fenitrothion, pirimiphos-methyl, Bendiocarb, bifenthrin, propoxur, Alphacypermethrin, cyfluthrin, Deltamethrin, etenfrox and lambda-cyhalothrin are the insecticide compounds and formulations recommended by WHO for IRS against malaria vectors. (WHO, 2009).

 

Pyrethroid insecticide resistance in the mosquito vectors is mainly associated with reduced target site sensitivity arising from a single point mutation in the sodium channel gene, often referred to as knockdown resistance (kdr). This resistance mechanism is widespread in West Africa and was reported for the first time in Nigeria in 2002 as cited by Awolola et al., (2007). However, information on resistance status of these vectors to the approved insecticides by WHO is lacking in Gombe State.

 

The selection of insecticides for the tests is line with the fact that they are part of the insecticides approved by WHO currently in use in Gombe State both as agrochemicals (pesticides) and domestic insecticides.

 

There have been reports of resistant strains of mosquitoes to different insecticides in Nigeria, hence the need to test those occurring in Gombe state, Nigeria. Resistance status of Culicine to Alphacypermethrin, Bifenthrin and Bendiocarb in Gombe state has not been ascertained or determined.

 

The study was limited to the collection of culicines mosquito larvae in three different locations in Gombe metropolis: Gombe state University, Dawaki quarters and new market area. The aim of the study was to determine the susceptibility status of culicine mosquitoes to Alphacypermethrin, Bifenthrin and Bendiocarb.

 

2 Study Area

Gombe State, is located in the northeastern part of Nigeria with its capital as Gombe city. It is located between latitude 9°30' and 12°30'N and longitudes 8°45' and 11°45'E of the Greenwich Meridian. It has a total land area of about 20,265 km2. It is bounded by Borno and Yobe to the north and east, Taraba and Adamawa to the south, and Bauchi to the west. The projected population from the 1996 National Census is estimated at 3,225,382 at annual growth rate of 3.2 percent (NPC, 2014). Gombe has two distinct climates, the dry season (November–March) and the rainy season (April–October) with an average rainfall of 850 mm. It is an agrarian state with about 60 percent of the population engaged in agriculture. Year round cultivation is possible in some parts of the state due to favourable weather and an extensive irrigation programme. A number of food and cash crops produced in the state include: Cereals: maize, millet, sorghum, rice and wheat; Legumes: Cowpeas, groundnuts, soya beans and bambara nuts. The vegetation of Gombe metropolis can be described as Sudan savannah with open grassland. Annon (2003).

 

3 Preliminary Investigation

A preliminary investigation was carried out within the study area to identify the breeding sites of culicines. This gave the information on areas in which the larvae of culicines mosquitoes were found.

 

4 Larval Collection Method

Larvae were collected from the identified breeding sites, using a ladle which was lowered into the water (breeding site) at an angle of about 45°C until one side is just below the surface of the water. While dipping, care was taken not to disturb the larvae which may cause them to swim downward. About 9-10 scoops were collected depending on the concentration of the larvae in the water in order to get enough quantity for rearing. The larvae collected were brought to the insect laboratory where they were reared to adults (WHO, 2008).

 

5 Rearing of the Larvae

All collected larvae were put into a hatching tray/larval bowl covered with a net and fed with a mixture of yeast and biscuits (10% yeast and 90% low fat biscuits). The adults emerge were transferred into the cage with an aspirator and fed with 10% glucose solution (WHO, 2008).

 

6 Susceptibility Test

Female non-blood feds mosquitoes from the cage were used exclusively as recommended by WHO (2008). The test was done inside the insect laboratory, each with a control. Both the test and the control mosquitoes were from the same source and belong to the same species.

 

In each of the holding tube, a clean white paper rolled into cylindrical shape was inserted. It was then fastened in position with a spring wire clip (silver for control, copper for test). Twenty (20) to 25 mosquitoes were introduced into the holding tubes using the aspirator provided. Mosquitoes were collected in lots of 10 and gently transferred to the holding tubes through the filling holes in each side. A pre-test holding period of one hour was necessary to guard against damage specimens, which were removed at the end of one hour period.

 

Impregnated paper was then introduced into the exposure tubes. Mosquitoes were then transferred into exposure tube by attaching it to the vacant screw top in the slide. The slide was pulled out to a point beyond the filling hole so that no part of it should block the tube opening; the mosquitoes were then blown gently down into the exposure tube. The slide was then closed. The holding tube was then detached and kept aside. The exposure tube was then left standing upright for the period of one hour; under moderate temperature and humidity. Knockdown mosquitoes were recorded at intervals of 5, 10, 20, 30, 40, 50, 60 minutes.

 

At the end of the required exposure period of one hour, both knockdown and live mosquitoes were transferred back to the holding tubes by reversing the procedure. The holding tube was set so that it stands on the side. A pad of moist cotton-wool containing sugar solution was placed on the screen. This was kept for 24 hours in a secluded place at room temperature. Final mortality counts were made 24 hour post exposure period. The result of dead and live one was recorded on the forms provided. Four replicate tests were done with each of the insecticides with control in each case.

 

The tests were carried out under standard laboratory condition for susceptibility test of insects at 25°C ± 2°C and 80% ± 10% relative humidity. 

 

7 Results

A total 1,500 female culicines mosquitoes (365 for each chemical and 135 for each control) were exposed to three different insecticide impregnated papers with Alphacypermethrin, bifenthrin and Bendiocarb with control in each case.

 

On exposure to the insecticides; mosquitoes from Gombe State University showed 96%, 63%, and 66% susceptibility to Alphacypermethrin, Bifenthrin and Bendiocarb respectively (Figure 1). The mosquito samples from Dawaki quarters indicated 92%, 62% and 69% susceptibility to Alphacypermethrin, Bifenthrin, and Bendiocarb respectively (Figure 2); whereas culicines mosquitoes from New market Area showed susceptibility of 90% to Alphacypermethrin, 44% and 69% to Bifenthrin and Bendiocarb respectively (Figure 3). No full susceptibility to any of the chemicals was observed; however, percentage mortality to Alphacypermethrin in all the three sites suggests the possibility of resistance that needs to be confirmed but results for Bifenthrin and Bendiocarb indicates resistance in all the study sites.

 

Applications of corrections to the mortality was not necessary as all the Control mosquitoes recorded below 5% mortality.

 

 

Figure 1 Susceptibility status of Culicines collected from Gombe State University Exposed to Alphacypermethrin (0.75%), Bifenthrin (0.15%) and Bendiocarb (0.1%) impregnated papers

 

 

Figure 2 Susceptibility status of Culicines collected from Dawaki Quarters Exposed to Alphacypermethrin (0.75%), Bifenthrin (0.15%) and Bendiocarb (0.1%) impregnated papers

 

 

Figure 3 Susceptibility status of Culicines collected from New Market Area Exposed to Alphacypermethrin (0.75%), Bifenthrin (0.15%) and Bendiocarb (0.1%) impregnated papers

 

8 Discussion

Mortality rate of female culicines mosquitoes from all the study areas exposed to Alphacypermethrin (a pyrethroid) were 96%, 92%, and 90%. This agrees with the work of Molta and Ali, (1998) in Northeastern Nigeria; where they reported 83.3%-89.3% mortality and 86.0% ± 2.9% corrected mortality of mosquitoes exposed to Permethrin (a pyrethroid)- impregnated papers using the WHO bioassay method.

 

Mortality of mosquitoes from all the study areas exposed to bifenthrin (a pyrethroid) impregnated papers indicated 63%, 62%, and 44% after 24 hours. Work of Chandre et al., (2000) in Burkina Faso and Cote d’ ivore also showed that nets impregnated with different pyrethroids provided good level of protection against mosquitoes. Adult female culicines from all the study areas exposed to Bendiocarb impregnated papers showed 66% and 69% mortality after 24 hours. This indicated a variation with the work of Martin et al., (2010) in Benin, West Africa which reported mortality rates of mosquitoes as 97.9% - 100% which showed susceptibility, the first month and 77% - 88% the third month on exposure to Bendiocarb. The local Anopheles mosquito species also were resistant Bifenthrin and Bendiocarb (Coetzee et al., 2006).

 

Several reports have indicated a higher incidence of insecticide resistance in areas of intensive agriculture than found in comparable non-agricultural sites (Diabate et al., 2002; Corbel et al., 2007; Yadouleton et al., 2009). There are also examples where use of pyrethroids for malaria control appears to be the primary factor in selecting for resistance (Stump et al, 2004; Czeher et al., 2008; John et al., 2008). Other sources of selection pressure including the use of consumer products such as coils or aerosols, or selection due to other toxins permeating mosquito breeding sites may also be contributing to the resistance phenotype (Choi et al., 2002; Djouaka et al., 2008).

 

Resistance observed in this study may be as a result of widespread utilization of chemical compounds against mosquitoes and other domestic insects and pests. Unpublished reports from the study area showed a high level usage of the chemicals under review against mosquitoes and other arthropod pests. 

 

9 Conclusions

Resistance observed in this study poses a threat to the current intervention measures against mosquito vectors (LLINs and IRS). The current findings will help in the choice of insecticide to use during campaigns of Indoor residual spraying in Gombe. Routine surveillance of insecticide susceptibility/resistance in Mosquito vector populations in the state and across Nigeria is necessary for effective resistance management strategy.

 

References

Annon, 2003, Gombe Master Plan, 2030. Savannah Land Development Consultant Nigeria Ltd. For Gombe State Government, Ministry of Land and Survey, pp.7-15

 

Awolola T.S., Oduola O.A., Oyewole I.O., Obansa J.B., Amajor C.N., Koekemoer L.L., and Coetzee M., 2007, Dynamic of Knockdown Pyrethroid Insecticide Resistance Alleles in a Field Population of Anopheles gambiae s.s. in South-Western Nigeria. p p. 50-57

 

Chandre F., Darriet F., Duchon S., Finot L., Manguin S., Carnevale P. and Guillet P., 2000, Modification of Pyrethroid Effect Associated with kdr Mutation in Anopheles gambiae, Medicaland Veterinary Entomology. 14: 81-88

http://dx.doi.org/10.1046/j.1365-2915.2000.00212.x

 

Choi J., Rose R.L., Hodgson E., 2002, In vitro Human Metabolism of Permethrin: the Role of Human Alcohol and Aldehyde dehydrogenase, Pesticide Biochemistry and Physiology, 73: 117-128

http://dx.doi.org/10.1016/S0048-3575(02)00154-2

 

Coetzee M., Van Wyk P., Booman M., Koekemoer L.L., Hunt R.H., 2006, Insecticide Resistance in Malaria Vector Mosquitoes in a Gold Mining Town in Ghana and Implications for Malaria Control, Bull. Soc. Pathol. Exot. 99(5): 400-403

 

Corbel V.N., Guessan R., Brengues C., Chandre F., Djogbenou L., Martin T., Akogbeto M., Hougard J.M., and Rowland M., 2007, Multiple Insecticide Resistance Mechanisms in Anopheles gambiae and Culex quinquefasciatus from Benin, West Africa, Acta Trop, 101(3): 207-216  

http://dx.doi.org/10.1016/j.actatropica.2007.01.005

 

Czeher C., Labbo R., Arzika I., Duchemin J.B., 2008, Evidence of increasing Leu-Phe Knockdown Resistance Mutation in Anopheles gambiae from Niger following a Nationwide Long-Lasting Insecticide-Treated Nets Implementation, Malar J, 7: 189

http://dx.doi.org/10.1186/1475-2875-7-189

 

Diabate A., Baldet T., Chandre F., Akoobeto M., Guiguemde T.R., Darriet F., Brengues C., Guillet P., Hemingway J., and Small G.J., 2002, The Role of Agricultural Use of Insecticides in Resistance to Pyrethroids in Anopheles gambiae s.l. in Burkina Faso, Am J Trop Med Hyg, 67(6): 617-622

 

Djouaka R.F., Bakare A.A., Coulibaly O.N., Akogbeto M.C., Ranson H., Hemingway J., and Strode C., 2008, Expression of the cytochrome P450s, CYP6P3 and CYP6M2 are Significantly Elevated in Multiple Pyrethroid Resistant Populations of Anopheles gambiae s.s. from Southern Benin and Nigeria. BMC Genomics, 9: 538

http://dx.doi.org/10.1186/1471-2164-9-538

 

George W.W., and David M.W., 2004, An Introduction to Insecticides, Meisterpro Information Resources, 4th Edition, pp.33 

 

John R., Ephraim T., and Andrew A., 2008, Reduced Susceptibility to Pyrethroid Insecticide Treated Nets by the Malaria Vector Anopheles gambiae s.l. in Western Uganda, Malar J, 7: 92

http://dx.doi.org/10.1186/1475-2875-7-92

 

Jordan E.L., and Verma P.S., 2007, Invertebrate Zoology, S. Chand and Company Ltd, pp.911-925

 

Kelly-Hope, Ransom L., and Hemingway J., 2008, Managing Insecticides Resistance Malaria Control and Eradication Programme, Malaria Journal, 8: 387-389

 

Kotpal R.L., 2010, Modern Textbook of Zoology- Invertebrates, Rastagi Publications, pp.93-95

 

Martin C. Akogbeto, Gil Germain Padonou, Dina Gbenou, Seth Irish and Agnes Yadouleton, 2010, Bendiocarb, a Potential Alternative Against Pyrethroid Resistant Anopheles gambiae in Benin, West Africa. Malaria Journal 9 (204): 1-9

http://dx.doi.org/10.1186/1475-2875-9-204

 

Molta N.B. and Ali A., 1998, Susceptibility of Anopheles Species of Northeastern Nigeria to Permethrin, Entomological Society of Nigeria, Occasional Publication 31: 101-107

 

National Population Commission (NPC Nigeria) and ICF International, 2014, Nigeria Demographic and Health Survey 2013

 

Stump A.D., Atieli F.K., Vulule J.M., and Besansky N.J., 2004, Dynamics of the pyrethroid knockdown resistance allele in western Kenyan populations of Anopheles gambiae in Response to Insecticide-treated Bed Net Trials, Am J Trop Med Hyg, 70 (6): 591-596

 

WHO, 2008, Protocols for Testing Mosquitoes Susceptibility to Insecticides Using Insecticides Test Kits and Impregnated Papers. pp. 5-8

 

WHO, 2009. WHO recommended Insecticides for Indoor Residual Spraying Against Malaria Vector. www.who.int/whopes

 

WHO, 2006 Newsletter. www.about.com

 

Yadouleton A.W., Asidi A., Djouaka R.F., Braima J., Agossou C.D., and Akogbeto M.C., 2009, Development of Vegetable Farming: a Cause of the Emergence of Insecticide Resistance in Populations of Anopheles gambiae in Urban Areas of Benin, Malar J, 8: 103

http://dx.doi.org/10.1186/1475-2875-8-103

 

Journal of Mosquito Research
• Volume 6
View Options
. PDF(493KB)
. FPDF
. HTML
. Online fPDF
Associated material
. Readers' comments
Other articles by authors
pornliz suckporn porndick pornstereo . E. Abba
. K. P.  Yoriyo
. J.  Philimon
. J. Ayuba
. B.S.  Abdulmalik
. H. Saidu
Related articles
. Susceptibility
. Alphacypermethrin
. Bifenthrin
. Bendiocarb
. Gombe
Tools
. Email to a friend
. Post a comment