Insecticide confrontation in Dengue vector: Enzymatic Characterization of tolerance level in the Mysore field populations of Aedes aegypti.  

Vasanth Patil H.B.1,2 , Nithin K S.3 , Sathish Kumar B.Y.1
1. Postgraduate Department of Studies and Research in Bio-technology, University of Mysore, JSS College, Ooty Road, Mysore – 570 025, INDIA
2. JSS Research Foundation (University of Mysore), Sri Jayachamarajendra College of Engineering, Manasagangothri, Mysore, INDIA
3. Postgraduate Department of Studies and Research in Chemistry, University of Mysore, JSS College, Ooty Road, Mysore – 570 025, INDIA

Author    Correspondence author
Journal of Mosquito Research, 2015, Vol. 5, No. 15   doi: 10.5376/jmr.2015.05.0015
Received: 30 Apr., 2015    Accepted: 02 Jun., 2015    Published: 29 Sep., 2015
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This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Vasanth Patil H.B., Nithin K.S., and Sathish Kumar B.Y., 2015, Insecticide Confrontation in Dengue Vector: Enzymatic Characterization of Tolerance Level in the Mysore Field Populations of Aedes aegypti, Journal of Mosquito Research, Vol.5, No.15 1-14 (doi: 10.5376/jmr.2015.05.0015)

Abstract

The susceptibility status of an insect population to any insecticide depends on several factors such includes genetic constitution, ecology of breeding place, previous history of insecticide application if any in that area and the cross resistance spectra. In view of the frequent outbreaks of dengue in different parts of the country and Karnataka state, in particular Mysore and surrounding districts, it is felt essential to generate a base line data on the susceptibility of vectors of local importance and their genetic differentiation through biochemical markers. Resistance to insecticides developed by Aedes aegypti was biochemically detected among its population collected from five different urban areas of Mysore city and from four rural locations of Mysore, Mandya and Hassan districts. Insect larvae exposed to different concentrations of insecticide - Deltamethrin for 24 hr. Insecticide resistance/tolerance level in terms of LC50 and LC90 for the insecticide was high in rural population than urban. Correspondingly, the reason for the resistance was detected through qualitative and quantitative analysis of three biochemical marker enzymes viz., A-Esterase, B-Esterase, Dehydrogenase (G6PD), and Phosphtases (Acid, Alkaline). The allelic frequency of Esterases and Phosphatases was more in rural over urban populations and the same was implied in quantitative estimation also. Wherein the allelic frequency of both the Phosphatases remains same in all the populations but the enzyme concentration was elevated in rural over the urban populations. The inspection of the present study reveals that, the Mysore populations of Ae. aegypti shows much variation for which their ecology was responsible.

Keywords
Deltamethrin; Esterases; G6PD-Glucose 6 phosphate dehydrogenase; Acid Phosphatase; Alkaline Phosphatase
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