Research Article

Laboratory Bioassay of Chilodonella uncinata, an Entomopathogenic Protozoan, against Mosquito Larvae  

Bina Pani Das1,3 , Kedar Deobhankar2 , Karuna N Pohekar3 , Rahul Marathe2 , Syed Akhtar Husain3 , P. Jambulingam4
1. National Centre for Disease Control, 22 Sham Nath Marg, Delhi 110054, India
2. Ross Lifescience Pvt. Ltd., Plot No 96, Sector no 10, PCNTDA, Bhosari, Pune 411026, India
3. Department of Biosciences, Jamia Millia Islamia University, New Delhi 110025, India
4. Vector Control Research Centre, Indira Nagar, Puducherry 605006, India
Author    Correspondence author
Journal of Mosquito Research, 2016, Vol. 6, No. 10   doi: 10.5376/jmr.2016.06.0010
Received: 19 Oct., 2015    Accepted: 18 Dec., 2015    Published: 27 Jan., 2016
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This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Das B.P., Deobhankar K., Pohekar K.N., Marathe R., Husain S.A., and Jambulingam P., 2016, Laboratory bioassay of Chilodonella uncinata, an entomopathogenic protozoan, against mosquito larvae, Journal of Mosquito Research, 6(10): 1-10 (doi: 10.5376/jmr.2016.06.0010)

Abstract
Background & objectives Use of microbial control agents provides alternative method for adequate insect management. We evaluated laboratory bioassay of Chilodonella uncinata, a natural protozoan parasite of mosquito larvae.
Methods Two formulations and four strains: North India (Monsoon and Pre-monsoon) strain, South India strain, updated strain of Chilodonella uncinata were tested against Anopheles stephensi, Culex quinquefasciatus and Aedes aegypti larvae at four institutes.
Results & Interpretation Delayed development was noted in mosquito larvae exposed to Ch. uncinata formulation that produced better effect in all larval species. Efficacy of this biolarvicide is not dose dependant as least dose produced maximum mortality with minimum post exposure. An. stephensi larvae were most sensitive followed by Cx. quinquefasciatus and Ae. aegypti which required longer post exposure. Of the four strains, updated strain was most effective and stable; south India strain had rapid killing effect; Pre-monsoon strain was more effective than Monsoon strain as the later required 20x higher dose (cs/ml) to induce satisfactory mortality in An. stephensi larvae with higher values for LT50 (4.71) and LT90 (6.84) as against LT50 (2.5) and LT90 (3.57) of former strain. Tea bag formulation is easy to store, transport and treat, was found to have a shelf life of >18 months resulting in satisfactory efficacy against An. stephensi with LT50 (5.16) and LT90 (7.69) noted at 0.25 g even after 6 months of storage. These laboratory test data suggest that a lower dose of this protozoan formulation can be used as a potential biolarvide to control mosquito larvae as an alternative to chemical insecticides under integrated vector management.
Keywords
Aedes aegypti; Anopheles stephensi; Chilodonella uncinata; Culex quinquefasciatus; Entomopathogenic protozoan; Mosquito larvicidal activity
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