Research Article

Phytochemical Analyses and Larvicidal Potentiality of Fruit Peel Extracts of Citrus limetta against Filarial Vector Culex quinquefasciatus   

Subrata Mallick1,2 , Utpal Adhikari1 , Anjali Rawani1,3 , Goutam Chandra1*
1 Department of Zoology, Mosquito, Microbiology and Nanotechnology Research Units, Parasitology Laboratory, The University of Burdwan, West Bengal,713104, India
2 Department of Zoology, Maharajadhiraj Uday Chand Womens' College, Burdwan, West Bengal, India
3 Department of Zoology, University of Gour Banga, Malda, West Bengal, 732103, India
Author    Correspondence author
Journal of Mosquito Research, 2016, Vol. 6, No. 22   doi: 10.5376/jmr.2016.06.0022
Received: 18 Jul., 2016    Accepted: 24 Nov., 2016    Published: 29 Dec., 2016
© 2016 BioPublisher Publishing Platform
This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Mallick S., Adhikari U., Rawani A., and Chandra G., 2016, Phytochemical analyses and larvicidal potentiality of fruit peel extracts of Citrus limetta against filarial vector Culex quinquefasciatus, 6(22): 1-7 (doi: 10.5376/jmr.2016.06.0022)

Abstract

Different concentrations of crude and different solvents viz., n-hexane, ethyl acetate, and methanol fruit peel extracts of Citrus limetta Risso (C. limetta) were taken for investigation for larvicidal activity against Culex quinquefasciatus Say, 1823 (Cx. quinquefasciatus) mosquito species. Crude extract showed good larvicidal activity with very low concentrations against 1st - 4th instars larvae. Only 3rd instar larvae were taken for larvicidal bioassay experiments for each solvent extract. LC50 values of n-hexane, ethyl acetate, and methanol fruit peel extracts of C. limetta were 661.27, 1268, and 939.43 ppm respectively after 24 h of exposure. No mortality was observed on control treatments. Log probit analyses, regression equations, and R2 values of larvicidal bioassay experiments with crude and different solvent extracts were determined. Statistical justification was done through ANOVA analyses. Qualitative phytochemical analyses were carried out and detected different secondary metabolites. No mortality was observed on tested non target organisms.      

Keywords
Citrus limetta; Culex quinquefasciatus; Larvicide; Non-target organisms; Phytochemicals
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