Molecular Phylogeney and Evolutionary Relationship among Four Mosquito (Diptera: Culicidae) Species from India Using PCR-RFLP  

Ajay Kumar Sharma , Chandel K. , Tyagi V. , Mendki M.J. , Tikar S.N. , Sukumaran D.
Vector Management Division, Defence R & D Establishment, Jhansi Road, Gwalior, Madhya Pradesh, India
Author    Correspondence author
Journal of Mosquito Research, 2013, Vol. 3, No. 8   doi: 10.5376/jmr.2013.03.0008
Received: 28 Feb., 2013    Accepted: 26 Mar., 2013    Published: 22 Apr., 2013
© 2013 BioPublisher Publishing Platform
This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Sharma et al., 2013, Molecular Phylogeney and Evolutionary Relationship among Four Mosquito (Diptera: Culicidae) Species from India Using PCR-RFLP, Journal of Mosquito Research, Vol 3, No.8 58-64 (doi: 10.5376/jmr.2013.03.0008)

Abstract

Mosquito of Culicidae (Diptera) family are known to transmit various diseases of public health importance. They are cosmopolitan in their distribution with diverse habitat and environmental conditions including in India. The generic and suprageneric relationships and the validity and monophyly of the generic and subgeneric groupings of Culicidae are in need of extensive reexamination. The application of explicit methods of phylogenetic analysis has revealed limitations in the conventional classification of mosquitoes. With the advent of molecular tools a little progress has been made, that provided a robust, stable classification that reflects evolutionary relationship. The present study is designed to establish the phylogenetic relation among the four different Culicidae mosquito species Anopheles stephensi, Aedes aegypti, Aedes albopictus and Culex quinquefasciatus by using Polymerase chain reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) of Cytochrome Oxidase I (COI) gene. The phylogenetic tree evolved using POPGENE software revealed phylogenetic relationship among them. The integration of DNA based methods foster the resolution of non-scientific but important uncertainty of nomenclature and also provide suitable means for delimiting and unambiguously identification of species for practical reasons including vector management.

Keywords
Culicidae; Mosquitoes; PCR-RFLP; Cytochrome oxidase-I
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