Research Report

Microsatellite markers linked to sterility mosaic disease resistance in pigeonpea (Cajanus cajan L. Millsp.)  

Prakash G. Patil1,2 , Byregowda M.1 , Bhuvaneshwara R. Patil3 , Alok Das2 , Mary Reena G.A.1 , Sowjanya M.S.1 , Shashidhar H.E.1
1 University of Agricultural Sciences, GKVK, Bengaluru-560 065, India
2 ICAR-Indian Institute of Pulses Research, Kanpur-208 024, India
3 University of Agricultural Sciences, Dharwad- 580 005, India
Author    Correspondence author
Legume Genomics and Genetics, 2016, Vol. 7, No. 6   doi: 10.5376/lgg.2016.07.0006
Received: 09 Mar., 2016    Accepted: 11 Apr., 2016    Published: 12 Jun., 2016
© 2016 BioPublisher Publishing Platform
This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Prakash G. Patil, Byregowda M., Bhuvaneshwara R. Patil, Alok Das, Mary Reena G.A., Sowjanya M.S., and Shashidhar H.E., 2016, Microsatellite markers linked to sterility mosaic disease resistance in pigeonpea (Cajanus cajan L. Millsp.), Legume Genomics and Genetics, 7(6): 1-9 (10.5376/lgg.2016.07.0006)

Abstract

Sterility mosaic disease (SMD) is a major viral disease of pigeonpea in southern India. In the present study, simple sequence repeat (SSR) markers were employed to identify those genomic regions associated with resistance to Bengaluru isolate of SMD through bulked segregant analysis (BSA) approach. Eighty four F6 recombinant inbred lines (RILs) derived from the cross ICP 8863 (susceptible) x BRG 3 (resistant) were used. A total of 50 hypervariable pigeonpea specific SSR primers (AHSSR) were screened for parental polymorphism study. Out of 50 primers surveyed, 40 primers (80%) were found polymorphic between parents. Based on BSA, three SSR markers viz., AHSSR 50150, AHSSR 34130 and AHSSR 20125 were successfully differentiated resistant and susceptible bulks, and also showed a clearcut differentiation between resistant and susceptible RIL individuals used for constituting these bulks. Segregation analysis of these three SSR markers on 84 F6 RILs exhibited expected Mendelian segregation ratio of 1:1. Single marker analysis (SMA) showed that AHSSR 50150, AHSSR 34130 and AHSSR 20125 were closely associated with SMD resistance and explained 21, 8 and 6 per cent of phenotypic trait variations. The markers identified from this could be useful for SMD resistance breeding in pigeonpea.

Keywords
Pigeonpea; Single marker analysis; Sterility mosaic disease; SSR markers
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