Cloning and Prokaryotic Expression of LHY Gene from Soybean
1 Institute of Life Sciences, Jiyang College of Zhejiang A&F University, Zhuji, 311800, China
2 Cuixi Academy of Biotechnology, Zhuji, 311800, China
3 Hainan Institute of Tropical Agricultural Resources, Sanya, 572025, China
4 College of Life Sciences and Technology, Guangxi University, Nanning, 530005, China
Legume Genomics and Genetics, 2017, Vol. 8, No. 2 doi: 10.5376/lgg.2017.08.0002
Received: 28 Feb., 2017 Accepted: 21 Mar., 2017 Published: 10 Apr., 2017
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Preferred citation for this article:
Li Z.F., Wu Z.Q., Liu Z.P., Wei F., Zhang J., Cai M.D., and Fang X.J., 2017, Cloning and prokaryotic expression of LHY Gene from soybean, Legume Genomics and Genetics, 8(2): 12-16 (doi: 10.5376/lgg.2017.08.0002)
LHY is the key gene of biological clock, we designed the corresponding primers which was based on soybean LHY candidate genes, we obtained the GmLHY2 gene by RT-PCR method to amplify the LHY genes in soybean cultivars. Its ORF span was 2250 bp, encoding was 749 amino acids. Then the GmLHY2 gene was built into PGEX expression vector to express the fusion protein of GST-GmLHY2, the prokaryotic expression condition of GST-GmLHY2 fusion protein was also explored. The result indicated that, with the help of comparative genomics between model plants and other plants, which can be more predictable and targeted in the study of other species.
Soybean; LHY Gene; RT-PCR; Expression vector; Comparative genomics
Legume Genomics and Genetics
• Volume 8