Research Report

Cloning and Prokaryotic Expression of LHY Gene from Soybean  

Zongfei Li1,2,3,4 , Zhongqi Wu1,2 , Zhenpeng Liu1,2 , Fang Wei1,2 , Jie Zhang1,2 , Mengdie Cai1,2 , Xuanjun Fang1,2,3,4
1 Institute of Life Sciences, Jiyang College of Zhejiang A&F University, Zhuji, 311800, China
2 Cuixi Academy of Biotechnology, Zhuji, 311800, China
3 Hainan Institute of Tropical Agricultural Resources, Sanya, 572025, China
4 College of Life Sciences and Technology, Guangxi University, Nanning, 530005, China
Author    Correspondence author
Legume Genomics and Genetics, 2017, Vol. 8, No. 2   doi: 10.5376/lgg.2017.08.0002
Received: 28 Feb., 2017    Accepted: 21 Mar., 2017    Published: 10 Apr., 2017
© 2017 BioPublisher Publishing Platform
This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Li Z.F., Wu Z.Q., Liu Z.P., Wei F., Zhang J., Cai M.D., and Fang X.J., 2017, Cloning and prokaryotic expression of LHY Gene from soybean, Legume Genomics and Genetics, 8(2): 12-16 (doi: 10.5376/lgg.2017.08.0002)

Abstract

LHY is the key gene of biological clock, we designed the corresponding primers which was based on soybean LHY candidate genes, we obtained the GmLHY2 gene by RT-PCR method to amplify the LHY genes in soybean cultivars. Its ORF span was 2250 bp, encoding was 749 amino acids. Then the GmLHY2 gene was built into PGEX expression vector to express the fusion protein of GST-GmLHY2, the prokaryotic expression condition of GST-GmLHY2 fusion protein was also explored. The result indicated that, with the help of comparative genomics between model plants and other plants, which can be more predictable and targeted in the study of other species.

Keywords
Soybean; LHY Gene; RT-PCR; Expression vector; Comparative genomics
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