Isolation and Expression Analysis of a Î²-Ketoacyl-Acyl Carrier Protein Synthase â… gene from Arachis hypogaea L.
Shandong Peanut Research Institute, Qingdao, 266100
Legume Genomics and Genetics, 2010, Vol. 1, No. 3 doi: 10.5376/lgg.2010.01.0003
Received: 01 Jul., 2010 Accepted: 10 Sep., 2010 Published: 10 Oct., 2010
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Preferred citation for this article:
Chi et al., 2010, Isolation and Expression Analysis of a β-Ketoacyl-Acyl Carrier Protein Synthase â… gene from Arachis hypogaea L., Legume Genomics and Genetics Vol.1 No.3 (doi: 10.5376/lgg.2010.01.0003)
β-ketoacyl-acyl carrier protein synthase (KAS) plays a pivotal role in de novo fatty acid biosynthesis, joining short carbon units to construct fatty acyl chains by a three-step Claisen condensation reaction in plants and bacteria. A putative KASâ… cDNAs was isolated from peanut by searching a peanut seedling full-length cDNA library. The AhKASâ… contains four strictly conserved residues Cys221–His361–Lys392–His397 in the active site, which is an important characteristic of KAS Is in plants and bacteria. The AhKASâ… gene containing 1 912 bp cDNA sequence with a 1 413 bp ORF, encodes 470 amino acids. The predicted amino acid sequences translated from the AhKASâ… gene shared 90.2% sequence identity to the corresponding protein in Glycine max. The cDNA was cloned into plasmid pET-28a and expressed in Escherichia coli BL21. Quantitative real-time PCR analysis suggested AhKASâ… was expressed with higher levels in leaf and seed than those in other tissues. In addition, AhKASâ… RNA was found in high abundance at 45 and 65 DAP (days after pegging) during seed development. This work may serve as a foundation for further studies on the mechanisms regulating the expression of KASâ… gene and provide candidate genes for modifying oil quality via transgenic plants.
Fatty acid biosynthesis; Î²-ketoacyl-ACP synthase â… ; Expression analysis; Peanut (Arachis hypogaea L.)