Colchicine-Induced Trait Variations in Azuki Bean (Vigna angularis)  

Baomei Wu , Hongxia Liu , Bo Zhao , Xing Tong , Qingmu Zhao , Ping Wan
Beijing University of Agriculture, College of Plant Science and Technology, Beijing, 102206
Author    Correspondence author
Legume Genomics and Genetics, 2010, Vol. 1, No. 7   doi: 10.5376/lgg.2010.01.0007
Received: 30 Aug., 2010    Accepted: 28 Sep., 2010    Published: 20 Oct., 2010
© 2010 BioPublisher Publishing Platform
This article was first published in Molecular Plant Breeding (Regular Print Version) in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Wan et al., 2009, Colchicine-induced Variations in Characters of Adzuki Bean, Molecular Plant Breeding, 7(6): 1169-1175 (doi: 10.3969/mpb.007.001169)

Abstract

Seeds of Jinnong 6 variety of azuki bean were soaked in 0.2% and 0.4% concentration of colchicines for 12 hours, respectively. The plant survival rates in M1 generation were 26.7% and 3.5%, and the variation rates were 1.24% and 1.59%, by which it was demonstrated that the higher the concentrations of colchicines, the lower the plant survival rate, and the greater the variation frequence.. The treatment of the 0.2% concentration of colchicines for 12h was optimal for the mutation of azuki bean, while more variation types were generated in the treatment of the 0.4% concentration. Compared with the control group, average number of seed per plant and 100-seed weight of between control and M2 generation with colchicine treatment showed significant difference at 5% and 1% levels, respectively; 100-seed weight of M3 generation had significant difference at 1% level. Three excellent lines with dominantly higher yield were gained. 263 mutants of leaf color, leaf shape, stem twining, more branch number, black pod, yellow-white seed, light red and deep seed color, large seed and late maturing in M3 generation were obtained. The variations of leaf shape involved sword, small heart-shaped leaf and kidney leaves. The variations of leaf color included dark green, light green, yellowing and yellow spot. Early-maturing mutants were earlier from 10 to 15 days and late-maturing mutants were later from 7 to 10 days than the control. The traits of sprawl, yellow-white seed, higher plant mutant can be steadily inherited in M4. The traits of kidney leaf , small heart-shape, sword leaf mutants are mostly inherited, Some mutants of glave kidney leaf in M3 separated sword, kidney, heart-shape leaves in M4.

Keywords
Azuki Bean (Vigna angularisi (Willd.) Ohwi & Ohashi ); Colchicine; Inducing-mutation; Mutant
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