A Mini-Review: Molecular Profiles of Diamondback Moth (Plutella xylostella)  

Jeffrey W. Lee
Insectarium, Saunders Institute at Fresno, CA, USA
Author    Correspondence author
Molecular Entomology, 2013, Vol. 4, No. 1   doi: 10.5376/me.2013.04.0001
Received: 24 Jan., 2013    Accepted: 28 Jan., 2013    Published: 19 Feb., 2013
© 2013 BioPublisher Publishing Platform
This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
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Lee J.W., 2013, A Mini-Review: Molecular Profiles of Diamondback Moth (Plutella xylostella), Molecular Entomology, Vol.4, No.1 1-5 (doi: 10.5376/me.2013.04.0001)

Abstract

Plutella xylostella (L.), also known as diamondback moth (DBM), is deemed to be a basal and primitive as well as highly heterozygous insect in the Plutellidae Family of Lepidoptera Order. Diamondback moth diverged about 124 million years ago from two other lepidopterans species, B. mori and D. plexippus. Diamondback moth has 31 chromosomes (n = 31) with a genome size of roughly 343Mb. Its genome consists of 18 071 protein-coding genes 781 non-coding RNAs, and repetitive sequences that represent 33.97% of the genome. In its genome, 1 412 genes are found to be unique to Diamondback moth. There are abundant DNA variations present in P. xylostella’s genome in the forms of SNPs, InDels, structural variations and complex segmental duplication patterns. DMB is able to adapt to a variety of environmental challenges as a result of preferential expression of a set of genes at the larval stage that contributes to odorant chemoreception, food digestion, metabolic detoxification, and in particular, a biological detoxification pathway in long-term evolution that is able to detoxify many chemicals including Bt toxins, thus making it a notorious lepidopteran pests.

Keywords
Diamondback moth (DBM); Plutella xylostella (L.); Genome; Molecular variation; Molecular evolution; Molecular adaptation

Plutella xylostella (L.) belongs to the family of Plutellidae in Lepidoptera Order, commonly known  Diamondback moth (DBM), because the adult male back forms three yellow diamonds at rest when the wings are folded (Ankersmit et al., 1953). Diamondback moth feeds on cruciferous plant, and it’s becoming one of the most intensively studied  Lepidopteran agricultural pests due to its devastating harm to the important cruciferous crops, as well as its resistance to many chemical pesticides and biological pesticides (Talekar and Shelton, 1993; Furlong et al., 2012). With the rapid development of modern biotechnology, a lot of studies have focused on the biological characteristics of diamondback moth at the molecular level, and significant progress is achieved regarding genomics and genetics, as well as molecular evolution and adaptation (Xie, 2013).

1 The genome of Diamondback moth
Diamondback moth has a genome size of about 343Mb, containing approximately about 18 071 protein encoding genes and 781 non-coding RNAs, and repetitive sequence (You et al., 2013). The diamondback moth has 1 412 genes unique to itself, most of which is involved in basic biological pathways of environmental information processing, chromosome replication or repair, transcriptional regulation, as well as carbohydrates, and protein metabolism (You et al., 2013).

2 Molecular genetics of diamondback moth
Diamondback moth is a highly heterozygous insect with 31chromosomes (n = 31). There are abundant DNA variations in genome such as SNPs, InDels and structural variation, as well as composite fragment repeats (Figure 1) (You et al., 2013). Baxter et al. built a linkage map of the diamondback moth by using next generation RAD sequencing technology (Baxter et al., 2011B). 3 177 maternally inherited RAD alleles were mapped on 31 chromosomes, making it possible to identify pesticide resistance genes and W/Z sex chromosome. The genome-wide linkage map spans 1 292 cM in length with 2 878 segregating RAD alleles inherited from the backcross father (Figure 2) (Baxter et al 2011). In the same year, Baxter also genetically mapped membrane transporter (ABCC2) to a locus contributing to Bt Cry1Ac toxin resistance in two lepidopteran insects, implying that this protein plays a crucial role in the Bt function (Baxter et al. 2011).

 

 

Figure 1 Genomic variations within the sequenced P. xylostella strain (adopted from You et al., 2013)

 

 

Figure 2 Linkage map of the Plutella xylostella (n = 31) genome (adopted from Baxter et al., 2011B)


3 Molecular evolution of Diamondback moth

Plutella xylostella (Linnaeus, 1758) belongs to the family Plutellidae of Lepidoptera order in the Insecta class, Arthropoda phylum and Animalia kingdom. Diamondback moth diverged from two other lepidopterans Bombyx mori and black monarch butterflies (D. plexippus) about 124 million years ago. Based on the existing insect genomic phylogeny,  diamondback moth was confirmed as a basal and primitive lepidopteran insect (You et al., 2013). Phylogenetic analysis further confirmed that the estimated divergence time of insecta orders was about 265~332 million years ago, which is consistent with the divergence of monocotyledonous and dicotyledonous plants 304 million years ago, indicating the co-evolution of insect behavior and host plant existence. When the cruciferous plants diverged from the Caricaceae about 54~90 million years ago, the diamondback moth also evolved to become a cruciferous plant-eating insects (You et al., 2013).

4 Molecular adaptation of diamondback moth
The diamondback moth originated in Europe. It was first found in North America in Illnois in 1854, and it spread to Florida and the Rocky Mountains in 1883. It was reported in British Columbia in 1905. Nowadays diamondback moth can be found in every corner of the planet where cabbage is planted (Furlong et al., 2012). Clearly, DMB has evolved to adapt to a variety of environmental challenges as a result of the complex network of genes preferentially expressed in the larval stage that contributes to odorant chemoreception, food digestion and metabolic detoxification.

DMB has become particularly resistant to chemical and biological pesticides in recent years. In the 1950s, Diamond moth was reported to have evolved resistance to DDT (Ankersmit et al., 1953), followed by a decline in the effectiveness of pyrethroid insecticides in the 1980s and reports of Diamond moth’s resistance to Bt toxins in the 1990s (Heckel et al., 1999; Tabashnik et al., 1999). Thereafter, virtually all kinds of pesticides have became ineffective. This adaption to insecticides contributes to the formation of biological detoxification pathways in long-term evolutionary process (You et al., 2013), and the ability to detoxify many chemical pesticides makes diamond moth a notorious lepidopteran pest.

References
Ankersmit G.W., 1953, DDT-resistance in Plutella maculipennis (Curt.) (Lep.) in Java, Bull. Entomol. Res., 44(3): 421-425
http://dx.doi.org/10.1017/S0007485300025530

Baxter Simon W., Francisco R. Badenes-Pérez, Anna Morrison, Heiko Vogel, Neil Crickmore, Wendy Kain, Ping Wang, David G. Heckel, and Chris D. Jiggins, 2011, Parallel evolution of Bacillus thuringiensis toxin resistance in Lepidoptera, Genetics, 189: 675-679
http://dx.doi.org/10.1534/genetics.111.130971 PMid:21840855 PMCid:3189815

Baxter S.W., Davey J.W., Johnston J.S., Shelton A.M., Heckel D.G., Jiggins C.D., and Blaxter M.L., 2011, Linkage Mapping and Comparative Genomics Using Next-Generation RAD Sequencing of a Non-Model Organism, PLoS ONE, 6(4): e19315
http://dx.doi.org/10.1371/journal.pone.0019315 

Furlong, M.J., Wright, D.J., and Dosdall L.M., 2007, Diamondback moth ecology and management: problems, progress, and prospects, Annu. Rev. Entomol., 58: 517-541
http://dx.doi.org/10.1146/annurev-ento-120811-153605

Heckel D.G., Gahan L.J., Liu Y.B., and Tabashnik B.E., 1999, Genetic mapping of resistance to Bacillus thuringiensis toxins in diamondback moth using biphasic linkage analysis, Proc. Natl. Acad. Sci. USA., 96(15): 8373-8377
http://dx.doi.org/10.1073/pnas.96.15.8373

Tabashnik B.E., Huang F.N., Ghimire M.N., Leonard B.R., Siegfried B.D., Murugesan Rangasamy, Yang Y.J., Wu Y.D., Gahan L.J., Heckel D.G., Alejandra Bravo, and Mario Soberón, 2011, Efficacy of genetically modified Bt toxins against insects with different genetic mechanisms of resistance, Nat. Biotechnol., 29: 1128-1131
http://dx.doi.org/10.1038/nbt.1988 PMid:21983521

Talekar N.S., and Shelton A.M., 1993, Biology, ecology, and management of the diamondback moth, Annu. Rev. Entomol., 38: 275-301
http://dx.doi.org/10.1146/annurev.en.38.010193.001423

Xie S., 2013, The Genome of Diamondback Moth Sequenced, a Model Pest in Lepidoptera, Jiyinzuxue Yu Shengwu Jishu (online), 2: 1-2
http://dx.doi.org/10.5376/gb.cn.2013.02.0001

You M.S., Yue Z., He W.Y., Yang X.H., Yang G., Xie M., Zhan D.L., Baxter S.W., Vasseur L., Gurr G.M., Douglas C.J., Bai J.L., Wang P., Cui K., Huang S.G., Li X.C., Zhou Q., Wu Z.Y., Chen Q.L., Liu C.H., Wang B., Li X.J., Xu X.F., Lu C.X., Hu M., Davey J.W., Smith S.M., Chen M.S., Xia X.F., Tang W.Q., Ke F.S., Zheng D.D., Hu Y.L., Song F.Q., You Y.C., Ma X.L., Peng L., Zheng Y.K., Liang Y., Chen Y.Q., Yu L.Y., Zhang Y.N., Liu Y.Y., Li G.Q., Fang L., Li J.X., Zhou X., Luo Y.D., Gou C.Y., Wang J.Y., Wang J., Yang H.M., and Wang J., 2013, A heterozygous moth genome provides insights into herbivory and detoxification, Nature Genetics, 45(2): 220-225
http://dx.doi.org/10.1038/ng.2524

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