Transformation of GBSS I Gene Driven with Barley Endosperm-specific Promoter into Maize Inbred Line Mediated by Agrobacterium  

Rongxi Zhou , Ying Wu , Hongda Zou , Shengzhong Su , Shipeng Li , Xiaohui Shan , Hongkui Liu , Yaping Yuan
College of Plant Science, Jilin University, Changchun, 130062, P.R. China
Author    Correspondence author
Maize Genomics and Genetics, 2012, Vol. 3, No. 1   doi: 10.5376/mgg.2012.03.0001
Received: 01 Dec., 2011    Accepted: 27 Dec., 2011    Published: 02 Jan., 2012
© 2012 BioPublisher Publishing Platform
This article was first published in Molecular Plant Breeding in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Zhou et al., 2012, Transformation of GBSS I  Gene Driven with Barley Endosperm-Specific Promoter into Maize Inbred Line Mediated by Agrobacterium, Maize Genomics and Genetics, Vol.3, No.1, 1-5 (doi: 10.5376/mgg.2012.03.0001)

Abstract

In present study, We made a plant expression construct that ligated GBSS I  cloned from common wheat and endosperm specific promoter HorD from barley into pCAMBIA3301 by using the two bridge vector pGM-T-HorD and pGM-T-GBSSI , then the construct named pHorD-GBSSI was transformed into EHA105. Somatic embryogenesis of inbred line Y423 was used to be transformed by Agrobacterium tumefaciens mediation. Regenerated plants were harvested to be genetically detected and total of 35 palnrts were positive by PCR accreditation, accounting for 15% . We thought this study might provide a molecular way to improve the quality of maize starch.

Keywords
Maize (Zea mays L.); Granule bound starch synthaseâ…  (GBSS I); Barley endosperm-specific promoter (HorD); Agrobacterium-mediated transformation
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