Construction of Plant Expression Vector with Maize γ-zein Gene and GFP Gene and Their Subcellular Localization
1 Academy of Sichuan Grassland Science, Chengdu, 611731, P.R. China;
2 Institute of Animal Science, Chinese Academy of Agricultural Science, Beijing, 100094, P.R. China
Maize Genomics and Genetics, 2012, Vol. 3, No. 2 doi: 10.5376/mgg.2012.03.0002
Received: 29 Feb., 2012 Accepted: 30 May, 2012 Published: 01 Jun., 2012
© 2012 BioPublisher Publishing Platform
This article was first published in Molecular Plant Breeding (2011, Vol.9, No.2, 174-179) in Chinese, and here was authorized to translate and publish the paper in English under the terms of Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:
Zhang et al., 2012, Construction of Plant Expression Vector with Maizeγ-zein gene and GFP gene and their Subcellular Localization, Maize Genomics and Genetics, Vol.3, No.2, 6-12 (doi: 10.5376/mgg.2012.03.0002)
The full length of the maize γ-zein gene amplified by PCR from PROK.TG1LK plasmid was ligated into the intermediate vector pMD18-T, to obtain the recombinant plasmid named pMD18-γ-zein. After the recombinant plasmid and the improved pCAMBIAI1302,respectively were digested by Nco I and Bgl II restriction endonucleases, the plant expression vector pCB-GFP-γ-zein with the gene of green fluorescent protein (GFP) driven by 35S was built following the procedures of recovery, ligation, transformation and identification. The fusion of GFP-γ-zein gene was integrated into the onion epidermal cells by using the Biolistic approach; the fusion gene was expressed in onion epidermal cell wall, nucleus and cytoplasm confirmed by confocal microscopy.
Maize; Î³-zein gene; Green fluorescent protein (GFP); Plant expression vector; Subcellular localization