Effect of the Leaf Litter of Transgenic Populus Simonii P. nigra on the Soil Microbial Community and Horizontal Transfer Possibility of the Foreign Gene
Syed Sadaqat Shah 2
1 School of Life Science, Northeast Forestry University, Harbin, 150040, China
2 Key Laboratory of Vegetation Ecology, Ministry of Education, Institute of Grassland Science, Northeast Normal University, Changchun 130024, China
Molecular Microbiology Research, 2017, Vol. 7, No. 1 doi: 10.5376/mmr.2017.07.0001
Received: 26 Jun., 2017 Accepted: 20 Jul., 2017 Published: 27 Jul., 2017
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This is an open access article published under the terms of the Creative Commons Attribution License
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Preferred citation for this article:
Peng M., Shah S.S., Wang Q.Y., and Meng F.J., 2017, Effect of the leaf litter of transgenic Populus simonii×P. nigra on the soil microbial community and horizontal transfer possibility of the foreign gene, Molecular Microbiology Research, 7(1): 1-9 (doi: 10.5376/mmr.2017.07.0001)
To study the potential influence of transgenic Populus simonii×P. nigra on the soil microbial community composition and horizontal exogenous gene transfer, fresh transgenic leaves were treated in broadleaf and coniferous forest soil for 6 months in the laboratory. The results showed that DNA fragments of the target and marker genes were completely degraded before 120 days. The dominant colony is the bacterium whose number is much more than that of other microbes under the same treatment. The number of cultivable bacteria treated by transgenic litters was significantly higher than the number treated by non-transgenic litters in selective medium with kanamycin. The target gene in the entophytic and soil bacteria, with 1.92% and 3.08% PCR-positive results, was found only in broadleaf forest soil. This result showed a certain possibility of horizontal gene transfer from the transgenic poplar to soil bacteria.
Transgenic poplar; Bt-the spider insecticidal peptide gene; NPTII gene; Soil microbe; Horizontal gene transfer
Molecular Microbiology Research
• Volume 7