Research Article

Effect of the Leaf Litter of Transgenic Populus Simonii P. nigra on the Soil Microbial Community and Horizontal Transfer Possibility of the Foreign Gene  

Mu Peng1 , Syed Sadaqat Shah 2 , Qiuyu Wang1 , Fanjuan  Meng1
1 School of Life Science, Northeast Forestry University, Harbin, 150040, China
2 Key Laboratory of Vegetation Ecology, Ministry of Education, Institute of Grassland Science, Northeast Normal University, Changchun 130024, China
Author    Correspondence author
Molecular Microbiology Research, 2017, Vol. 7, No. 1   doi: 10.5376/mmr.2017.07.0001
Received: 26 Jun., 2017    Accepted: 20 Jul., 2017    Published: 27 Jul., 2017
© 2017 BioPublisher Publishing Platform
This is an open access article published under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Preferred citation for this article:

Peng M., Shah S.S., Wang Q.Y., and Meng F.J., 2017, Effect of the leaf litter of transgenic Populus simonii×P. nigra on the soil microbial community and horizontal transfer possibility of the foreign gene, Molecular Microbiology Research, 7(1): 1-9 (doi: 10.5376/mmr.2017.07.0001)

Abstract

To study the potential influence of transgenic Populus simonii×P. nigra on the soil microbial community composition and horizontal exogenous gene transfer, fresh transgenic leaves were treated in broadleaf and coniferous forest soil for 6 months in the laboratory. The results showed that DNA fragments of the target and marker genes were completely degraded before 120 days. The dominant colony is the bacterium whose number is much more than that of other microbes under the same treatment. The number of cultivable bacteria treated by transgenic litters was significantly higher than the number treated by non-transgenic litters in selective medium with kanamycin. The target gene in the entophytic and soil bacteria, with 1.92% and 3.08% PCR-positive results, was found only in broadleaf forest soil. This result showed a certain possibility of horizontal gene transfer from the transgenic poplar to soil bacteria.

Keywords
Transgenic poplar; Bt-the spider insecticidal peptide gene; NPTII gene; Soil microbe; Horizontal gene transfer
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